Citation
Bahrun D., . and Rashid A. A., . and Kadir M. A., . and Aziz M. A., . (2004) Callus induction and establishment of suspension culture from seed derived cotyledon of cabbage cv. 864 Green Storm. [Proceedings Paper]
Abstract
Callus of cabbage Brassica oleracea var. capitata L. were initiated from cotyledon explants derived from seeds. Cotyledon explants cultured on medium containing 8 and 10 mgL- 2 4-D resulted in high intensity of callus formation with 75-100 of the explant surface covered with callus. The callus produced in these treatments was friable whitish and translucent. On medium without 2 4-D no callus formation was observed. Upon transfer of the friable callus to liquid MS medium containing 1 mgL- 2 4-D rounded cells with dense cytoplasm embryogenic were obtained.
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Abstract
Callus of cabbage Brassica oleracea var. capitata L. were initiated from cotyledon explants derived from seeds. Cotyledon explants cultured on medium containing 8 and 10 mgL- 2 4-D resulted in high intensity of callus formation with 75-100 of the explant surface covered with callus. The callus produced in these treatments was friable whitish and translucent. On medium without 2 4-D no callus formation was observed. Upon transfer of the friable callus to liquid MS medium containing 1 mgL- 2 4-D rounded cells with dense cytoplasm embryogenic were obtained.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
| AGROVOC Term: | Seeds |
| AGROVOC Term: | Brassica oleracea capitata |
| AGROVOC Term: | Cabbages |
| AGROVOC Term: | Cotyledons |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Callus culture |
| AGROVOC Term: | Seed germination |
| AGROVOC Term: | Cytoplasm |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12825 |
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