Citation
Panjaitan S. B., . and Aziz M. A., . and Rashid A. A., . and Saleh N. M., . (2004) Somatic embryogenesis from petiole explant of papaya Carica papaya L. Cv. Eksotika. [Proceedings Paper]
Abstract
An experiment was carried out to induce somatic embryogenesis from petiole explants of papaya cultivar Eksotika using different concentrations and combinations of 2.4-D and BAP. Petiole explants were cultured on modified MS medium with half-strength macro nutrients and supplemented with 500 mgL- glutamine 0.2 phytagel and 1.0 2.5 5.0 and 7.5 mgL- 2 4-D in combination with 0 0.001 0.005 and 0.010 mgL- BAP. Treatment containing 7.5 mgL- 2 4-D without BAP produced the highest percentage of callus formation 89. Highest percentage of somatic embryo formation 20 occurred in treatment containing 5.0 mgL- 2 4-D. In this treatment the mean number of somatic embryos produced per explant was 12.
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Abstract
An experiment was carried out to induce somatic embryogenesis from petiole explants of papaya cultivar Eksotika using different concentrations and combinations of 2.4-D and BAP. Petiole explants were cultured on modified MS medium with half-strength macro nutrients and supplemented with 500 mgL- glutamine 0.2 phytagel and 1.0 2.5 5.0 and 7.5 mgL- 2 4-D in combination with 0 0.001 0.005 and 0.010 mgL- BAP. Treatment containing 7.5 mgL- 2 4-D without BAP produced the highest percentage of callus formation 89. Highest percentage of somatic embryo formation 20 occurred in treatment containing 5.0 mgL- 2 4-D. In this treatment the mean number of somatic embryos produced per explant was 12.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
| AGROVOC Term: | Carica papaya |
| AGROVOC Term: | Papayas |
| AGROVOC Term: | Petioles |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Experiments |
| AGROVOC Term: | Somatic embryogenesis |
| AGROVOC Term: | Callus culture |
| AGROVOC Term: | Callus |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12826 |
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