Multiplication of oil palm Elaeis guinensis L. cultures in bioreactors

Tarmizi A. H., . and Samsul K. R., . (2004) Multiplication of oil palm Elaeis guinensis L. cultures in bioreactors. [Proceedings Paper]

The bioreactor technique was developed in order to further improve the liquid culture system of oil palm. Work at MPOB revealed that oil palm liquid cultures could be multiplied in a B-Braun ;Biostat B; 2L version 1.0 bioreactor. Some modifications were made to the bioreactor. After modifications some cultures showed good proliferation with about 10- to 14-fold weight increment after 50 to 80 days. This bioreactor system thus has the potential for large scale multiplication of oil palm suspension cultures in a single run with efficient utilization of time and space. Cultures were also multiplied in Sixfors Multi Fermenter System Infors Bottmingen Switzerland. The bioreactor with the working volume of 300 to 500 ml was suitable for small scale production and can also be used to study the behavior of cultures in response to nutrient and physical conditions. The fresh weight of cultures from clone E107 increased about 10-fold and cultures which has higher biomass utilized carbon sources faster. These findings on bioreactor will further enhance progress towards a semi or fully-automated process of oil palm clonal production.

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Abstract

The bioreactor technique was developed in order to further improve the liquid culture system of oil palm. Work at MPOB revealed that oil palm liquid cultures could be multiplied in a B-Braun ;Biostat B; 2L version 1.0 bioreactor. Some modifications were made to the bioreactor. After modifications some cultures showed good proliferation with about 10- to 14-fold weight increment after 50 to 80 days. This bioreactor system thus has the potential for large scale multiplication of oil palm suspension cultures in a single run with efficient utilization of time and space. Cultures were also multiplied in Sixfors Multi Fermenter System Infors Bottmingen Switzerland. The bioreactor with the working volume of 300 to 500 ml was suitable for small scale production and can also be used to study the behavior of cultures in response to nutrient and physical conditions. The fresh weight of cultures from clone E107 increased about 10-fold and cultures which has higher biomass utilized carbon sources faster. These findings on bioreactor will further enhance progress towards a semi or fully-automated process of oil palm clonal production.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palm
AGROVOC Term:	Plant multiplication
AGROVOC Term:	Bioreactors
AGROVOC Term:	Liquidity
AGROVOC Term:	Suspension culture
AGROVOC Term:	Nutrient elements
AGROVOC Term:	Physical states
AGROVOC Term:	Utilization
AGROVOC Term:	Clonal variation
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12841

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