Cryopreservation of oil palm Elaeis guineensis zygotic embryos using the vitrification technique

Uma Rani Sinniah, . (2004) Cryopreservation of oil palm Elaeis guineensis zygotic embryos using the vitrification technique. [Proceedings Paper]

Oil palm Elaeis guineensis is one of the main plantation crops in Malaysia. Due to its importance it is vital that a broad genetic base is conserved for future improvement. Oil palm germplasm has been mainly conserved in field genebanks due to its recalcitrant seed properties. Field conservation is costly to maintain and require much land and management to control pests and diseases. For long-term conservation the use of in vitro techniques especially cryopreservation has been most promising. Cryopreservation is the storage of materials under ultra-low temperature of liquid nitrogen. Various methods of cryopreservation are available but this study evaluates the potential of using vitrification technique as opposed to the desiccation technique on survival of cryopreserved oil palm embryos. The type of loading and vitrification solution used often influences the vitrification process and is species specific. Time of exposure to both loading and vitrification solution also plays as important role as prolonged exposure will result in toxicity. In this study seven loading solutions were tested and the best loading solution was tested for response to exposure time. Subsequently the effect of five vitrification solution was tested. The best survival percentage obtained was compared with the survival obtained using the desiccation method. It appears that the vitrification technique is not appropriate for oil palm as only around 10 survival was obtained while circa 50 survival can be obtained using the desiccation technique.

Full text available from:

Abstract

Oil palm Elaeis guineensis is one of the main plantation crops in Malaysia. Due to its importance it is vital that a broad genetic base is conserved for future improvement. Oil palm germplasm has been mainly conserved in field genebanks due to its recalcitrant seed properties. Field conservation is costly to maintain and require much land and management to control pests and diseases. For long-term conservation the use of in vitro techniques especially cryopreservation has been most promising. Cryopreservation is the storage of materials under ultra-low temperature of liquid nitrogen. Various methods of cryopreservation are available but this study evaluates the potential of using vitrification technique as opposed to the desiccation technique on survival of cryopreserved oil palm embryos. The type of loading and vitrification solution used often influences the vitrification process and is species specific. Time of exposure to both loading and vitrification solution also plays as important role as prolonged exposure will result in toxicity. In this study seven loading solutions were tested and the best loading solution was tested for response to exposure time. Subsequently the effect of five vitrification solution was tested. The best survival percentage obtained was compared with the survival obtained using the desiccation method. It appears that the vitrification technique is not appropriate for oil palm as only around 10 survival was obtained while circa 50 survival can be obtained using the desiccation technique.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palm
AGROVOC Term:	Vitrification
AGROVOC Term:	Vitrification plant
AGROVOC Term:	Germplasm
AGROVOC Term:	Germplasm conservation
AGROVOC Term:	Cryopreservation
AGROVOC Term:	Plant embryos
AGROVOC Term:	Desiccation treatment
AGROVOC Term:	Survival
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12843

Actions (login required)

View Item