Citation
Asma Dazni, . and Julia Ibrahim, . and Shamsul Hairi Salleh, . and Khor Bee Gek, . and Azlina Aziz, . and Ooi Wai Ling, . and Tan Ann Na, . and Mohd Amiron, . and Abdul Kadir Mohamed, . and Mohd Zairey Azwan Mohd Zain, . (2008) Evaluation of antimicrobial and anticancer activities of plants from Gunung Nuang Forest Reserve. [Proceedings Paper]
Abstract
A preliminary study was carried out on cold ethanolic extracts of the plants collected from Gunung Nuang forest Selangor. The extracts were subjected to antimicrobial screening against a total of nine panel organisms comprising six non-pathogenic microbes and three pathogenic microbes. ADT assay method was applied whereby the appearance of an inhibition zone would indicate the presence of antimicrobial activity. Cytotoxcity testing was conducted by screening the crude plant extracts against human breast cancer cell lines MCF-7 using an MIT assay method. Tamoxifen an anticancer drug was used as a positive control as a comparison. In situ alkaloids test was also carried out. 4 plants species showed positive alkaloid tests. While with the ADT assays II extracts produced strong activities towards one or more target organisms. The MIT assay results suggested that 18 plant species contained compounds that are cytotoxic to MCF-7 cells. Out of the total of 62 plant specimens 3 species showed very significant activities in both assays. The 3 species are P100 P150 and P154 and they showed strong inhibition against MRSA S138 Escherichia coli 0157:H7 and VRE. Further investigations will be carried out on these promising plant extracts.
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Abstract
A preliminary study was carried out on cold ethanolic extracts of the plants collected from Gunung Nuang forest Selangor. The extracts were subjected to antimicrobial screening against a total of nine panel organisms comprising six non-pathogenic microbes and three pathogenic microbes. ADT assay method was applied whereby the appearance of an inhibition zone would indicate the presence of antimicrobial activity. Cytotoxcity testing was conducted by screening the crude plant extracts against human breast cancer cell lines MCF-7 using an MIT assay method. Tamoxifen an anticancer drug was used as a positive control as a comparison. In situ alkaloids test was also carried out. 4 plants species showed positive alkaloid tests. While with the ADT assays II extracts produced strong activities towards one or more target organisms. The MIT assay results suggested that 18 plant species contained compounds that are cytotoxic to MCF-7 cells. Out of the total of 62 plant specimens 3 species showed very significant activities in both assays. The 3 species are P100 P150 and P154 and they showed strong inhibition against MRSA S138 Escherichia coli 0157:H7 and VRE. Further investigations will be carried out on these promising plant extracts.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Uni-versiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal RM666 H33 B573 Call Number |
| AGROVOC Term: | Plants |
| AGROVOC Term: | Forest reserves |
| AGROVOC Term: | Antimicrobial properties |
| AGROVOC Term: | Cancer disease |
| AGROVOC Term: | Angiospermae |
| AGROVOC Term: | Evaluation |
| AGROVOC Term: | Plant extracts |
| AGROVOC Term: | Precipitation |
| AGROVOC Term: | Staphylococcus aureus |
| AGROVOC Term: | Escherichia coli |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12847 |
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