Effects of Strobilanthes crispus juice on activity of superoxide and dismutase and glutathione peroxidase in normal and streptozotocin-induced diabetic rats

Faridah H., . and Asmah R., . and Fauziah O., . and Norfarizan Hanoon N. A., . and Rokiah M. Y., . (2008) Effects of Strobilanthes crispus juice on activity of superoxide and dismutase and glutathione peroxidase in normal and streptozotocin-induced diabetic rats. [Proceedings Paper]

Increased oxidative stress has been reported in patients with diabetes mellitus. Accumulating evidence suggests that oxidative cellular injury caused by free radicals contributes to the development of diabetes mellitus. Reactive oxygen species generated in the cells are scavenged by antioxidant enzymes. Moreover diabetes also induces changes in the tissue content and activity of the antioxidant enzymes. This study was to determine the effect of Strobilanthes crispus juice on the activity of antioxidant enzymes such as superoxide dismutase SOD and glutathione peroxidase GPx in normal and streptozotocin-induced diabetic male albino Sprague dawley rats. This study was conducted on normal and streptozotocin-induced diabetic male Sprague dawley rats fed with basal diet and Strobilanthes crispus juice with difference doses 0.5 0.75 and 1.0 ml/kg body weight for 21 days. A total of 54 male albino Sprague dawley rats 24 normal and 30 streptozotocin-induced diabetic rats weight 150 -200 g were used. The rats were divided into 9 groups with 6 rats in each group. SOD and GPx were determined at day 0 baseline and day 21 by fully-automated spectrophotometric method. The results showed that the activity of SOD decreased significantly p 0.05 compared with baseline data both in normal and diabetic group. A significant decrease in SOD activity were found in diabetic rats treated with 0.5 m1/kg b.w S.crispus juice 0.86 0.04 units/L 0.75 m1/kg b.w S.crispus juice 0.89 0.28units/Land 1.0 ml/kgb.w S.crispus juice 0.87 0.08units/L. Significant decrease of SOD was also observed in the normal rats treated with 0.5 m1/kg b.w S.crispus juice 0.74 0.26 units/l 0.75 ml/kg b.w S.crispus juice 1.00 0.80 units/L and 1.0 m1/kg b.w S.crispus juice 0.91 0.21 units/L. SOD activity in diabetic rats treated with S.crispus juice 0.5 0.75 and 1.0 ml/kg b.w was also low compared in diabetic control. GPx activity significantly increased p 0.05 in diabetic rats treated with 0.75 and 1.0 ml/kg b.w S.crispus juice 2506.14 49.96 and 2701.96 414.51 units/ml when compared to diabetic control group 1914.88 270.68 units/mI. GPx activity has no significant difference between treated and non treated in normal group. In conclusion S. crispus juice which contains catechins alkaloids caffeine and tannin may have a protective role for both GPx and SOD by replacing or compensating their activities in removing hydrogen peroxide and scavenging superoxide free radicals respectively.

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Abstract

Increased oxidative stress has been reported in patients with diabetes mellitus. Accumulating evidence suggests that oxidative cellular injury caused by free radicals contributes to the development of diabetes mellitus. Reactive oxygen species generated in the cells are scavenged by antioxidant enzymes. Moreover diabetes also induces changes in the tissue content and activity of the antioxidant enzymes. This study was to determine the effect of Strobilanthes crispus juice on the activity of antioxidant enzymes such as superoxide dismutase SOD and glutathione peroxidase GPx in normal and streptozotocin-induced diabetic male albino Sprague dawley rats. This study was conducted on normal and streptozotocin-induced diabetic male Sprague dawley rats fed with basal diet and Strobilanthes crispus juice with difference doses 0.5 0.75 and 1.0 ml/kg body weight for 21 days. A total of 54 male albino Sprague dawley rats 24 normal and 30 streptozotocin-induced diabetic rats weight 150 -200 g were used. The rats were divided into 9 groups with 6 rats in each group. SOD and GPx were determined at day 0 baseline and day 21 by fully-automated spectrophotometric method. The results showed that the activity of SOD decreased significantly p 0.05 compared with baseline data both in normal and diabetic group. A significant decrease in SOD activity were found in diabetic rats treated with 0.5 m1/kg b.w S.crispus juice 0.86 0.04 units/L 0.75 m1/kg b.w S.crispus juice 0.89 0.28units/Land 1.0 ml/kgb.w S.crispus juice 0.87 0.08units/L. Significant decrease of SOD was also observed in the normal rats treated with 0.5 m1/kg b.w S.crispus juice 0.74 0.26 units/l 0.75 ml/kg b.w S.crispus juice 1.00 0.80 units/L and 1.0 m1/kg b.w S.crispus juice 0.91 0.21 units/L. SOD activity in diabetic rats treated with S.crispus juice 0.5 0.75 and 1.0 ml/kg b.w was also low compared in diabetic control. GPx activity significantly increased p 0.05 in diabetic rats treated with 0.75 and 1.0 ml/kg b.w S.crispus juice 2506.14 49.96 and 2701.96 414.51 units/ml when compared to diabetic control group 1914.88 270.68 units/mI. GPx activity has no significant difference between treated and non treated in normal group. In conclusion S. crispus juice which contains catechins alkaloids caffeine and tannin may have a protective role for both GPx and SOD by replacing or compensating their activities in removing hydrogen peroxide and scavenging superoxide free radicals respectively.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Uni-versiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal RM666 H33 B573 Call Number
AGROVOC Term:	Acanthaceae
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Antioxidants
AGROVOC Term:	Blood sugar
AGROVOC Term:	Diabetes
AGROVOC Term:	Chemical composition
AGROVOC Term:	Enzymes
AGROVOC Term:	Enzyme activity
AGROVOC Term:	Animal models
AGROVOC Term:	Leaves
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12868

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