Citation
Chin Y. W., . and Mamat A. S., . and Kubulat K. H., . (2000) The effect of PP333 and Gibberellins on growth and development of Roselle. [Proceedings Paper]
Abstract
The qualitative detection of the presence of two types endogenous hormone Auxin lAA and Abscisic Acid ABA was carried out and the effects of exogenous Gibberelin and Paclobutrazol treatment on the content of ABA in the calyx of Roselle was studied. The Ultraviolet-Visible Spectrophotometry and Infrared Spectrophotometry method were applied in detecting the presence of IAA. Whilst only the Ultraviolet-Visible Spectrometry method was used in detecting the presence of ABA. The Ultraviolet-Visible spectrums of both GA3 and PP333 treated calyx samples showed a presence of IAA and ABA. Nonetheless peak shifts were detected in both cases. This maybe due to variables such as solvent shifts and the substitution of other electron accepting or donating functional groups. Thus the Infrared Spectrometry was used to further confirm the specific functional groups present in both samples. The Infrared spectrums of GA3 and PP333 showed the presence of a carboxylic acid group a nitrile group and an aromatic ring which is consistent with the structure of IAA. In conclusion the presence of both IAA and ABA were detected in the GA3 and PP333 treated calyx samples. It was also observed that there is a positive correlation between the concentration of PP333 and the content of ABA. On the contrary there is a negative correlation between the concentration of GA3 and the content of ABA. PP333 at 500 ppm significally increased the number of fruits from week 7 to week 13 during harvesting period.
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Abstract
The qualitative detection of the presence of two types endogenous hormone Auxin lAA and Abscisic Acid ABA was carried out and the effects of exogenous Gibberelin and Paclobutrazol treatment on the content of ABA in the calyx of Roselle was studied. The Ultraviolet-Visible Spectrophotometry and Infrared Spectrophotometry method were applied in detecting the presence of IAA. Whilst only the Ultraviolet-Visible Spectrometry method was used in detecting the presence of ABA. The Ultraviolet-Visible spectrums of both GA3 and PP333 treated calyx samples showed a presence of IAA and ABA. Nonetheless peak shifts were detected in both cases. This maybe due to variables such as solvent shifts and the substitution of other electron accepting or donating functional groups. Thus the Infrared Spectrometry was used to further confirm the specific functional groups present in both samples. The Infrared spectrums of GA3 and PP333 showed the presence of a carboxylic acid group a nitrile group and an aromatic ring which is consistent with the structure of IAA. In conclusion the presence of both IAA and ABA were detected in the GA3 and PP333 treated calyx samples. It was also observed that there is a positive correlation between the concentration of PP333 and the content of ABA. On the contrary there is a negative correlation between the concentration of GA3 and the content of ABA. PP333 at 500 ppm significally increased the number of fruits from week 7 to week 13 during harvesting period.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number |
| AGROVOC Term: | Roselle |
| AGROVOC Term: | Hibiscus sabdariffa |
| AGROVOC Term: | Ultraviolet spectrophotometry |
| AGROVOC Term: | Infrared spectrophotometry |
| AGROVOC Term: | Paclobutrazol |
| AGROVOC Term: | Abscisic acid |
| AGROVOC Term: | Auxins |
| AGROVOC Term: | Gibberellins |
| AGROVOC Term: | Plant hormones |
| AGROVOC Term: | Plant developmental stages |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12886 |
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