Citation
Norzulaani Khalid, . (2000) Tissue culture and transgenic plants : success hopes and vision. [Proceedings Paper]
Abstract
The main purpose for carrying out tissue culture studies in our laboratory is for cloning transformation studies and the production of new lines through polyploidisation and somaclonal variation. For cloning purposes crops such as banana ginger citrus and musk melon have been developed. To date banana Musa spp. varieties such as mas cinta manis a mas variant berangan rastali lemak manis have been successfully cloned for commercial purposes. Currently meristem explants have been adopted with success. To enhance production and to ensure high quality product innovative equipment have been developed and alternative explants with high regeneration capacity have been employed. This paper will discuss both innovations in the production of tissue culture banana plants. An attempt to produce new variants of banana through long term cultures and field assessments are currently being carried out. We have now selected a few lines with superior agronomic traits and now recloning them via tissue culture before reassessment in the field to ensure stability. As for the medicinal ginger family following successful cloning experiments variants will be induced through polyploidisation of the ginger rhizomes using mutagens. We have now successfully produced transgenic brinjal with PVY genes and now evaluating the gen stability in the Fl generation. We hope in the near future to produce transgenic chilli plants with CMV and pungent genes tobacco with dengue virus protein and banana with fungal resistance. In this paper we will discuss how we trouble shoot problems encountered in certain experiments and the alternative solutions being adopted. Transient assay studies of various crops will be discussed in the absence of stable gene integration. The future for obtaining successful transgenic crops seems promising with the development of efficient tissue culture and the availability of suitable construct.
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Abstract
The main purpose for carrying out tissue culture studies in our laboratory is for cloning transformation studies and the production of new lines through polyploidisation and somaclonal variation. For cloning purposes crops such as banana ginger citrus and musk melon have been developed. To date banana Musa spp. varieties such as mas cinta manis a mas variant berangan rastali lemak manis have been successfully cloned for commercial purposes. Currently meristem explants have been adopted with success. To enhance production and to ensure high quality product innovative equipment have been developed and alternative explants with high regeneration capacity have been employed. This paper will discuss both innovations in the production of tissue culture banana plants. An attempt to produce new variants of banana through long term cultures and field assessments are currently being carried out. We have now selected a few lines with superior agronomic traits and now recloning them via tissue culture before reassessment in the field to ensure stability. As for the medicinal ginger family following successful cloning experiments variants will be induced through polyploidisation of the ginger rhizomes using mutagens. We have now successfully produced transgenic brinjal with PVY genes and now evaluating the gen stability in the Fl generation. We hope in the near future to produce transgenic chilli plants with CMV and pungent genes tobacco with dengue virus protein and banana with fungal resistance. In this paper we will discuss how we trouble shoot problems encountered in certain experiments and the alternative solutions being adopted. Transient assay studies of various crops will be discussed in the absence of stable gene integration. The future for obtaining successful transgenic crops seems promising with the development of efficient tissue culture and the availability of suitable construct.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number |
| AGROVOC Term: | Crops |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Micropropagation |
| AGROVOC Term: | Genetic transformation |
| AGROVOC Term: | Varieties |
| AGROVOC Term: | Induced polyploidization |
| AGROVOC Term: | Somaclonal variation |
| AGROVOC Term: | Bananas |
| AGROVOC Term: | Ginger |
| AGROVOC Term: | Citrus |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12888 |
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