Citation
M. Y. Omar, . and M. A. Latif, . and S. G. Tan, . (2000) DALP Direct Amplification Of Length Polymorphism: a PCR based DNA fingerprinting method in brown planthopper Nilaparvata lugens STAL Homoptera : Delphacidae. [Proceedings Paper]
Abstract
DALP is a new DNA fingerprinting method based on the use of arbitrarily primed PCR AP-PCR to selectively amplify discrete fragments of DNA from the genome of any species which are ready to be sequenced to provide information on different polymorphic loci without having to do cloning. This method was found to be useful in brown planthopper N. lugens. An oligonucleotide pair DALP235 and DALPR;5 was tested on two sympatric populations of brown planthopper N. lugens one from rice Oryza sativa and the other from a weed grass Leersia hexandra to study the genetic variation between the two populations. This oligonucleotide pair produced polymorphic codominant bands. These codominant bands may be used for the development of microsatellite primers for the brown planthopper N. lugens.
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Abstract
DALP is a new DNA fingerprinting method based on the use of arbitrarily primed PCR AP-PCR to selectively amplify discrete fragments of DNA from the genome of any species which are ready to be sequenced to provide information on different polymorphic loci without having to do cloning. This method was found to be useful in brown planthopper N. lugens. An oligonucleotide pair DALP235 and DALPR;5 was tested on two sympatric populations of brown planthopper N. lugens one from rice Oryza sativa and the other from a weed grass Leersia hexandra to study the genetic variation between the two populations. This oligonucleotide pair produced polymorphic codominant bands. These codominant bands may be used for the development of microsatellite primers for the brown planthopper N. lugens.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number |
| AGROVOC Term: | Nilaparvata lugens |
| AGROVOC Term: | Plant hoppers |
| AGROVOC Term: | Delphacidae |
| AGROVOC Term: | Homoptera |
| AGROVOC Term: | DNA fingerprinting |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Electrophoresis |
| AGROVOC Term: | Autoradiography |
| AGROVOC Term: | Polymorphism |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12891 |
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