Isolation and expression of a heat shock protein gene during ripening and storage of papaya fruit

Razean Haireen Mohd Razali, . and Zainon Mohd Ali, . and Hamid Lazan, . (2000) Isolation and expression of a heat shock protein gene during ripening and storage of papaya fruit. [Proceedings Paper]

Postharvest heat treatment which may induce chilling injury tolerance in papaya Carica papaya L cv Eksotika may possibly affect the expression of the different class of heat shock protein HSPs. In this study a cDNA library prepared from ripening papaya fruits were screened at reduced stringency with pTOM66 a clone encoding small molecular size tomato smHSP. A positive cDNA clone of about 1.5 kb in length was isolated and analyzed and showed homology to an unknown protein of Arabidopsis thaliana. Expression of this hypothetical papaya HSP carHSP gene during normal ripening and storage of papaya at chilling temperature 10C was investigated by Northern blotting. Results indicate that expression of carHSP decreased through normal ripening at ambient but the levels of the mRNA transcripts particularly in those fruits that have been subjected to heat-treatment increased during storage at low temperature LT. Transferring the LT-stored fruits to ambient resulted in an initial suppression of carHSP expression. but later the gene activity seemed to recover.

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Abstract

Postharvest heat treatment which may induce chilling injury tolerance in papaya Carica papaya L cv Eksotika may possibly affect the expression of the different class of heat shock protein HSPs. In this study a cDNA library prepared from ripening papaya fruits were screened at reduced stringency with pTOM66 a clone encoding small molecular size tomato smHSP. A positive cDNA clone of about 1.5 kb in length was isolated and analyzed and showed homology to an unknown protein of Arabidopsis thaliana. Expression of this hypothetical papaya HSP carHSP gene during normal ripening and storage of papaya at chilling temperature 10C was investigated by Northern blotting. Results indicate that expression of carHSP decreased through normal ripening at ambient but the levels of the mRNA transcripts particularly in those fruits that have been subjected to heat-treatment increased during storage at low temperature LT. Transferring the LT-stored fruits to ambient resulted in an initial suppression of carHSP expression. but later the gene activity seemed to recover.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number
AGROVOC Term:	Papayas
AGROVOC Term:	Carica papaya
AGROVOC Term:	Fruits
AGROVOC Term:	Isolation
AGROVOC Term:	Gene expression
AGROVOC Term:	Heat shock proteins
AGROVOC Term:	Genes
AGROVOC Term:	Ripening
AGROVOC Term:	Crop storage
AGROVOC Term:	Heat treatment
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12896

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