Isolation of A cDNA encoding cytosolic phosphoglucomutase from ripening chilli fruit

Wizana Zainal Abidin, . and Zainon Mohd Ali, . and Zamri Zainal, . (2000) Isolation of A cDNA encoding cytosolic phosphoglucomutase from ripening chilli fruit. [Proceedings Paper]

Phosphoglucomutase PGM EC 5.4.2.2 which catalyzes the interconversion of glucose 1- and glucose 6-phosphate is one of the enzymes that may be involved in the maintenance of equilibrium concentrations of relevant sugars at the hexose monophosphate HMP junction. This HMP junction which include also the interconversion of fructose-6-phosphate and glucose-6-phosphate represents important points in the metabolic pathways of cellular carbohydrates and other cellular components. We have isolated and partially sequenced a cDNA clone encoding cytosolic phosphoglucomutase from the library of ripening chilli Capsicum annuum L. cv MCII and designated capPGMl. This cDNA clone shows high homology at the deduced amino acid level with the cytosolic form of PGM from Solanum tuberosum 95 Arabidopsis thaliana 83 Pisum sativum 84 and cambium of poplar Populus tremula L. x P. tremuloides L. 83. Northern blot analysis seems to indicate that capPGMl was expressed throughout ripening.

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Abstract

Phosphoglucomutase PGM EC 5.4.2.2 which catalyzes the interconversion of glucose 1- and glucose 6-phosphate is one of the enzymes that may be involved in the maintenance of equilibrium concentrations of relevant sugars at the hexose monophosphate HMP junction. This HMP junction which include also the interconversion of fructose-6-phosphate and glucose-6-phosphate represents important points in the metabolic pathways of cellular carbohydrates and other cellular components. We have isolated and partially sequenced a cDNA clone encoding cytosolic phosphoglucomutase from the library of ripening chilli Capsicum annuum L. cv MCII and designated capPGMl. This cDNA clone shows high homology at the deduced amino acid level with the cytosolic form of PGM from Solanum tuberosum 95 Arabidopsis thaliana 83 Pisum sativum 84 and cambium of poplar Populus tremula L. x P. tremuloides L. 83. Northern blot analysis seems to indicate that capPGMl was expressed throughout ripening.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number
AGROVOC Term:	Chillies
AGROVOC Term:	Capsicum annuum
AGROVOC Term:	Isolation
AGROVOC Term:	Phosphoglucomutase
AGROVOC Term:	Ripening
AGROVOC Term:	Glucose 6 phosphate dehydrogenase
AGROVOC Term:	DNA
AGROVOC Term:	Genes
AGROVOC Term:	Solanum tuberosum
AGROVOC Term:	Arabidopsis thaliana
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12897

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