Characterisation and antigenicity of the outer membrane protein of Brucella melitensis

lIIazuwa Mohd Yusoff, . and Md Sabri Mohd. Yusoff, . and Siti Khairani Bejo, . (2011) Characterisation and antigenicity of the outer membrane protein of Brucella melitensis. [Proceedings Paper]

Brucella melitensis is the most pathogenic species infecting human and may cause abortions in goats sheep and cows. This disease is called brucellosis affecting various domestic and wild life species resulting from an infection of bacteria that belong to the genus Brucella. Among the bacterial components the outer membrane proteins OMPs are particularly attractive for the development of vaccine or diadnostic kits. In this study the OMPs profiles of local isolates of B. melitensis were determined using Sodium Oodecyl Sulfate-Polyacrylamide Gel Electrophoresis SDS-PAGE. This study also aimed at determining of the antigenicity of B. melitensis OMPs using Western immunoblotting method. Four major OMPs were successfully evaluated from the local B. melitensis isolates and results showed that 94 kDa major OMPs of B. melitensis were strongly antigenic to the antisera against B. melitensis produced in rabbit.

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Abstract

Brucella melitensis is the most pathogenic species infecting human and may cause abortions in goats sheep and cows. This disease is called brucellosis affecting various domestic and wild life species resulting from an infection of bacteria that belong to the genus Brucella. Among the bacterial components the outer membrane proteins OMPs are particularly attractive for the development of vaccine or diadnostic kits. In this study the OMPs profiles of local isolates of B. melitensis were determined using Sodium Oodecyl Sulfate-Polyacrylamide Gel Electrophoresis SDS-PAGE. This study also aimed at determining of the antigenicity of B. melitensis OMPs using Western immunoblotting method. Four major OMPs were successfully evaluated from the local B. melitensis isolates and results showed that 94 kDa major OMPs of B. melitensis were strongly antigenic to the antisera against B. melitensis produced in rabbit.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Brucella melitensis
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Brucellosis
AGROVOC Term:	Brucella
AGROVOC Term:	Evaluation
AGROVOC Term:	Infection
AGROVOC Term:	Protein isolates
AGROVOC Term:	Antigens
AGROVOC Term:	Immunoblotting
AGROVOC Term:	Animal diseases
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12908

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