Citation
Che Radziah C. M. Z., . and Sreeramanan S., . and Puspita Deswina, . and Marziah M., . (2000) Identification of the target corm meristematic tissue from shoot proliferation of banana cultivars. [Proceedings Paper]
Abstract
The banana is a large perennial herbaceous plant. It consists of an underground stem known as corm. Corm tissues are amenable to genetic transformation because they contain a large percentage of actively dividing regenerable cells. Berangan was identified to have the greatest potential for commercial cultivation and the elite Berangan selection was known as Intan. In this experiment Berangan Intan and Mas the popular local dessert cultivars were used. The aim of this study was to identify the target corm tissues which produced the highest number of shoots in 4 weeks. Corm slices of 2mm thick and 5-8mm diameter numbered I to 5 were cultured onto Murashige Skoog 1962 medium containing 5 mg/l BAP. Cultures grown under a 24 hour photoperiod were provided with white fluorescent at 27C. Corm tissues started to swell after I week of culture and were transferred to fresh medium after 2 weeks. The shoots were formed and the average number of shoots produced per corm slice was always highest in slice number 2 followed by number 3. The maximum number of shoots per corm slice obtained were 12 shoots in all cultivars examined Intan Berangan and Mas. Corm slice number 2 obtained from this study could be a potential source for establishing transformation protocols for our future investigations.
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Abstract
The banana is a large perennial herbaceous plant. It consists of an underground stem known as corm. Corm tissues are amenable to genetic transformation because they contain a large percentage of actively dividing regenerable cells. Berangan was identified to have the greatest potential for commercial cultivation and the elite Berangan selection was known as Intan. In this experiment Berangan Intan and Mas the popular local dessert cultivars were used. The aim of this study was to identify the target corm tissues which produced the highest number of shoots in 4 weeks. Corm slices of 2mm thick and 5-8mm diameter numbered I to 5 were cultured onto Murashige Skoog 1962 medium containing 5 mg/l BAP. Cultures grown under a 24 hour photoperiod were provided with white fluorescent at 27C. Corm tissues started to swell after I week of culture and were transferred to fresh medium after 2 weeks. The shoots were formed and the average number of shoots produced per corm slice was always highest in slice number 2 followed by number 3. The maximum number of shoots per corm slice obtained were 12 shoots in all cultivars examined Intan Berangan and Mas. Corm slice number 2 obtained from this study could be a potential source for establishing transformation protocols for our future investigations.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number |
| AGROVOC Term: | Bananas |
| AGROVOC Term: | Musa bananas |
| AGROVOC Term: | Cultivars |
| AGROVOC Term: | In vitro regeneration |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Corms |
| AGROVOC Term: | Meristems |
| AGROVOC Term: | Shoots |
| AGROVOC Term: | Micropropagation |
| AGROVOC Term: | Plant propagation |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12939 |
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