Early detection of green-fluorescent protein in plantain banana Musa spp. via particle bombardment


Citation

Deswina P., . and Marziah M., . and Maheran Abd. Aziz, . and Nor; Aini Mohd. Fadzillah, . (2000) Early detection of green-fluorescent protein in plantain banana Musa spp. via particle bombardment. [Proceedings Paper]

Abstract

Genetic transformation of Musa spp. cv. Nangka using the green fluorescent protein GFP gene as a marker gene was developed the explants used were callus and rhizome slices. Bombardment parameters were optimized for high levels of transient expression of the gfp gene and exhibiting the results were recorded 72 hours after bombardment. Efficiency of the transformation was based on the gfp-fluorescent spots in callus and rhizome slices. The results obtained showed that 90 of culture exhibited gfp-fluorescence spots with parameter of 1100 rupture disk pressure. The target distance utilised was 12 cm for callus and 6 cm for rhizome slices. Using 1350 psi disk pressure and 12 cm target distance the highest efficiency was 80 for rhizome slices and 60 for callus. The gfp-expressing spots in callus could be detected after 3 months of bombardment however from the rhizome slices it could be achieved after regeneration 2 months. Visualisation of GFP fluorescent in plant tissues was achieved under the Leica MZFLIII Stereo Microscope and also from photographs taken using a Nikon F60D automatic exposure system.


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Abstract

Genetic transformation of Musa spp. cv. Nangka using the green fluorescent protein GFP gene as a marker gene was developed the explants used were callus and rhizome slices. Bombardment parameters were optimized for high levels of transient expression of the gfp gene and exhibiting the results were recorded 72 hours after bombardment. Efficiency of the transformation was based on the gfp-fluorescent spots in callus and rhizome slices. The results obtained showed that 90 of culture exhibited gfp-fluorescence spots with parameter of 1100 rupture disk pressure. The target distance utilised was 12 cm for callus and 6 cm for rhizome slices. Using 1350 psi disk pressure and 12 cm target distance the highest efficiency was 80 for rhizome slices and 60 for callus. The gfp-expressing spots in callus could be detected after 3 months of bombardment however from the rhizome slices it could be achieved after regeneration 2 months. Visualisation of GFP fluorescent in plant tissues was achieved under the Leica MZFLIII Stereo Microscope and also from photographs taken using a Nikon F60D automatic exposure system.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number
AGROVOC Term: Musa sapientum
AGROVOC Term: Bananas
AGROVOC Term: Musa plantains
AGROVOC Term: Musaceae
AGROVOC Term: Genetic transformation
AGROVOC Term: Explants
AGROVOC Term: Callus
AGROVOC Term: Rhizomes
AGROVOC Term: Proteins
AGROVOC Term: Plant establishment
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/12941

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