Citation
Tan Yee How, . and Naderali Neda, . and Ganesan Vadamalai, . and Wong Mui Yun, . (2011) Genetic diversity of Lasiodiplodia theobromae infecting Jatropha curcas in Peninsular Malaysia determined with ISSR-PCR. [Proceedings Paper]
Abstract
Lasiodiplodia theobromae is a pathogen causing stem canker and dieback disease resulting in a high mortality rate and up to 50 yield loss in Jatropha curcas holdings in Malaysia. Jatropha is cited as important for biofuel production. The extent of genetic diversity among 20 isolates of Lasiodiplodia theobromae infecting Jatropha plantations in Peninsular Malaysia was examined using inter simple sequence repeat ISSR primers. Seven primers were tested for amplification of the twenty isolates of which only four primers ISSR4 ISSR6 ISSR8 and UBC835 created reproducible and interpretable amplification products. The four primers produced a total of 483 amplified bands of which 126 bands were polymorphic. The percentage of polymorphic DNA fragments ranged from 22.6 to 29.9. The primer sequence which was most commonly amplified was AGn. Jaccard;s genetic similarity index was employed to construct a dendrogram and nine clusters obtained. Based on the clusters observed there was no correlation between genotype and location. ISSR markers provided a quick reliable and informative way for establishing the genetic diversity of the pathogen.
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Abstract
Lasiodiplodia theobromae is a pathogen causing stem canker and dieback disease resulting in a high mortality rate and up to 50 yield loss in Jatropha curcas holdings in Malaysia. Jatropha is cited as important for biofuel production. The extent of genetic diversity among 20 isolates of Lasiodiplodia theobromae infecting Jatropha plantations in Peninsular Malaysia was examined using inter simple sequence repeat ISSR primers. Seven primers were tested for amplification of the twenty isolates of which only four primers ISSR4 ISSR6 ISSR8 and UBC835 created reproducible and interpretable amplification products. The four primers produced a total of 483 amplified bands of which 126 bands were polymorphic. The percentage of polymorphic DNA fragments ranged from 22.6 to 29.9. The primer sequence which was most commonly amplified was AGn. Jaccard;s genetic similarity index was employed to construct a dendrogram and nine clusters obtained. Based on the clusters observed there was no correlation between genotype and location. ISSR markers provided a quick reliable and informative way for establishing the genetic diversity of the pathogen.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Jatropha curcas |
| AGROVOC Term: | Euphorbiaceae |
| AGROVOC Term: | Lasiodiplodia theobromae |
| AGROVOC Term: | Fungi |
| AGROVOC Term: | Fungal diseases |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Gel electrophoresis |
| AGROVOC Term: | Polymerase chain reaction |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12947 |
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