Citation
Nurul Azlyn Azmi, . and Sharifah Aminah Syed Mmohamad, . and Yeoh No Na, . and Ramlan Mohamed, . (2011) Distribution of Pasteurella multocida genotype among animal cases in Malaysia. [Proceedings Paper]
Abstract
P. multocida was commonly characterized according to the established five capsular types designated as A B D F and F. In this study P. multocida strains isolated from 121 animal cases and confirmed by phenotyping analysis were used in genotype distribution study in Malaysia. Multiplex PCR assay effectively characterized individual capsular type for all P. multocida isolates by production of two distinct products; KMT1 amplimer as species specific identification and the individual specific capsular type of each isolates. Multiplex PCR identified four common P. multocida capsular types in Malaysia which are capsular type A in 38 31.4 cases type B in 23 19.0 cases type D in 59 48.8 cases and only 1 0.8 case belonged to type F. Based on the genotypic distribution among animal cases most avian 12/18 and rabbit 4/5 cases were caused by capsular type A cattle 7/16 and buffalo 3/5 cases were capsular type B and 48/66 of caprine cases were capsular type D. In conclusion P. multocida isolated from animal cases in Malaysia is dominated by capsular type D followed by capsular types A and B.
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Abstract
P. multocida was commonly characterized according to the established five capsular types designated as A B D F and F. In this study P. multocida strains isolated from 121 animal cases and confirmed by phenotyping analysis were used in genotype distribution study in Malaysia. Multiplex PCR assay effectively characterized individual capsular type for all P. multocida isolates by production of two distinct products; KMT1 amplimer as species specific identification and the individual specific capsular type of each isolates. Multiplex PCR identified four common P. multocida capsular types in Malaysia which are capsular type A in 38 31.4 cases type B in 23 19.0 cases type D in 59 48.8 cases and only 1 0.8 case belonged to type F. Based on the genotypic distribution among animal cases most avian 12/18 and rabbit 4/5 cases were caused by capsular type A cattle 7/16 and buffalo 3/5 cases were capsular type B and 48/66 of caprine cases were capsular type D. In conclusion P. multocida isolated from animal cases in Malaysia is dominated by capsular type D followed by capsular types A and B.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol. 2 Call Number. |
| AGROVOC Term: | Pasteurella |
| AGROVOC Term: | Pasteurella multocida |
| AGROVOC Term: | Gram negative bacteria |
| AGROVOC Term: | Genotypes |
| AGROVOC Term: | Genes |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Wild animals |
| AGROVOC Term: | Domestic animals |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12956 |
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