Isolation and characterisation of NBS-LRR motif based resistance genes in rice

Kalaivani Nadarajah, . and Ong Shin Tze, . (2011) Isolation and characterisation of NBS-LRR motif based resistance genes in rice. [Proceedings Paper]

The presence of a single resistance R gene allele can determine plant disease resistance. The protein products of such genes may act as receptors that specifically interact with pathogen-derived factors. Most functionally defined R-genes are of the nucleotide binding site-leucine rich repeat NBS-LRR supergene family and are present as large multigene families. The specificity of R-gene interactions together with the robustness of plant-pathogen interactions raises the question of their gene number and diversity in the genome. Genomic sequences from rice showing significant homology to genes conferring race specific resistance to pathogens were identified by systematically scanning the genome using a variety of primer pairs based on ubiquitous NBS motifs. Over 70 sequences were isolated and 10 are putative pseudogenes. Phylogenetic clustering of R-gene homologues within rice and other graminaceae family members was observed but not with R gene homologues from Arabidopsis thaliana. Our data indicates remarkably rapid evolution of R-gene homologues during diversification of plant families.

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Abstract

The presence of a single resistance R gene allele can determine plant disease resistance. The protein products of such genes may act as receptors that specifically interact with pathogen-derived factors. Most functionally defined R-genes are of the nucleotide binding site-leucine rich repeat NBS-LRR supergene family and are present as large multigene families. The specificity of R-gene interactions together with the robustness of plant-pathogen interactions raises the question of their gene number and diversity in the genome. Genomic sequences from rice showing significant homology to genes conferring race specific resistance to pathogens were identified by systematically scanning the genome using a variety of primer pairs based on ubiquitous NBS motifs. Over 70 sequences were isolated and 10 are putative pseudogenes. Phylogenetic clustering of R-gene homologues within rice and other graminaceae family members was observed but not with R gene homologues from Arabidopsis thaliana. Our data indicates remarkably rapid evolution of R-gene homologues during diversification of plant families.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Oryza
AGROVOC Term:	Oryza sativa
AGROVOC Term:	Genes
AGROVOC Term:	Molecular genetics
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	PCR
AGROVOC Term:	Phylogeny
AGROVOC Term:	DNA
AGROVOC Term:	Gene sequence
AGROVOC Term:	Genomes
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12960

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