Identification of diazotrophs isolated from Tanjong Karang rice growing area

Naher Umme Aminun, . and Radziah Othman, . (2011) Identification of diazotrophs isolated from Tanjong Karang rice growing area. [Proceedings Paper]

An in vitro study was conducted to identify the rice root associated diazotrophs isolated from Tanjong Karang rice irrigation project area. Samples were collected from both soil and plant grown on seven soil series or types Bakau Bernam Jawa Sedu Serong Brown clay Organic Clay and Muck and Brown Clay. Diazotrophs populations were higher in rhizosphere compared to shoot and root endophytes. Ten diazotrophs that performed high acetylene reduction assay were identified using 16SrRNA partial gene sequence analysis and identified as Bacillus spp. The genetic relatedness data showed that the isolates were from four major groups and sub-groups. The study of root colonisation using scanning electron microscope SEM and transmission electron microscope TEM proved that diazotrophs were able to colonise the surface and the interior of roots. In general tropical soils of Tanjong Karang rice irrigation project area harbor different species of Bacillus which form natural association with rice plant.

Full text available from:

Abstract

An in vitro study was conducted to identify the rice root associated diazotrophs isolated from Tanjong Karang rice irrigation project area. Samples were collected from both soil and plant grown on seven soil series or types Bakau Bernam Jawa Sedu Serong Brown clay Organic Clay and Muck and Brown Clay. Diazotrophs populations were higher in rhizosphere compared to shoot and root endophytes. Ten diazotrophs that performed high acetylene reduction assay were identified using 16SrRNA partial gene sequence analysis and identified as Bacillus spp. The genetic relatedness data showed that the isolates were from four major groups and sub-groups. The study of root colonisation using scanning electron microscope SEM and transmission electron microscope TEM proved that diazotrophs were able to colonise the surface and the interior of roots. In general tropical soils of Tanjong Karang rice irrigation project area harbor different species of Bacillus which form natural association with rice plant.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Diazotrophs
AGROVOC Term:	Bacteria
AGROVOC Term:	Nitrogen fixing bacteria
AGROVOC Term:	Root colonization
AGROVOC Term:	Identification
AGROVOC Term:	Isolation
AGROVOC Term:	Roots
AGROVOC Term:	Oryza sativa
AGROVOC Term:	Rice fields
AGROVOC Term:	Microbes
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12966

Actions (login required)

View Item