Copper tolerant bacteria and fungi isolated from copper contaminated agricultural soil

Che Fauziah I., . and Siti Nazurah Mohd Kamel, . and Radziah Othman, . (2011) Copper tolerant bacteria and fungi isolated from copper contaminated agricultural soil. [Proceedings Paper]

This study aimed to isolate and identify potential copper-tolerant fungi and bacteria from copper contaminated vegetable sites. Soils samples were analysed for Cu content using aqua regia digestion together with corresponding plants samples using nitric acid digestion. Fungal and bacterial strains were isolated from soil and surfacesterilised plant roots. Copper tolerance was measured by in vivo assay using dilution technique and isolates were identified based on 16S rDNA gene sequence analysis. The Minimum Inhibitory Concentrations MICs ranged from 50 to 300 mg L depending on the isolates which belonged to the genera Trichoderma UPMF2 Penicillium UPMF3 Fusarium UPMF4 Aspergillus UPMF5 T.viride UPMF6 Bacillus spp. UPMB2 UPMB7 Bacillus cereus UPMB4 Burkhoderia sp. UPMB5 Seratia marcesens UPMB6 and Bacillus mojavensis UPMB8. Bacillus sp. UPMB7 and T. viride UPMF6 isolates were the most Cu tolerant and exhibited strong growth. The merits of these strains as potential candidates for bioremediation require further investigations.

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Abstract

This study aimed to isolate and identify potential copper-tolerant fungi and bacteria from copper contaminated vegetable sites. Soils samples were analysed for Cu content using aqua regia digestion together with corresponding plants samples using nitric acid digestion. Fungal and bacterial strains were isolated from soil and surfacesterilised plant roots. Copper tolerance was measured by in vivo assay using dilution technique and isolates were identified based on 16S rDNA gene sequence analysis. The Minimum Inhibitory Concentrations MICs ranged from 50 to 300 mg L depending on the isolates which belonged to the genera Trichoderma UPMF2 Penicillium UPMF3 Fusarium UPMF4 Aspergillus UPMF5 T.viride UPMF6 Bacillus spp. UPMB2 UPMB7 Bacillus cereus UPMB4 Burkhoderia sp. UPMB5 Seratia marcesens UPMB6 and Bacillus mojavensis UPMB8. Bacillus sp. UPMB7 and T. viride UPMF6 isolates were the most Cu tolerant and exhibited strong growth. The merits of these strains as potential candidates for bioremediation require further investigations.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Bacteria
AGROVOC Term:	Fungi
AGROVOC Term:	Soil fertility
AGROVOC Term:	Soil sampling
AGROVOC Term:	Isolation
AGROVOC Term:	Identification
AGROVOC Term:	Contaminated soils
AGROVOC Term:	Agricultural soils
AGROVOC Term:	Gene sequence
AGROVOC Term:	Fusarium
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12967

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