Citation
Nurul Azyla Azmi, . and Sharifah Aminah Syed Mohamad, . and Ramlan Mohamed, . (2011) Analysis of IROMPs production in Pasteurella multocida 6:B from cattle. [Proceedings Paper]
Abstract
Haemorrhagic Septicaemia HS is a major disease of cattle and buffaloes characterised by an acute highly fatal septicaemia with high morbidity and mortality. In Asia HS is caused by Pasteurella multocida serotype B:2 or 6:B. The production of iron regulated outer membrane proteins IROMPs is regulated by Fur protein through repression of the expression of the respective genes when complexed with iron. In this study outer membrane proteins OMPs and iron-regulated outer membrane proteins IROMPs were prepared by extraction with 1.5 sarcosyl from the strain cultured on BHI medium and on a medium supplemented with different concentration of 2 2;-dipyridyl. Outer membrane proteins from a Pasteurella multocida 6:B local isolate were characterised by using sodium dodecyl sulphate polyacrylamide gel electrophoresis SDS-PAGE technique. The approximate size of each OMPs detected were determined from log of molecular weight and Rf value. A total of 12 polypeptides ranging from 19.2 to 117.4 kDa were observed including 33 and 37kDa major OMPs of Pasteurella multocida and two other 117.4 and 91 kDa prominent protein bands.
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Abstract
Haemorrhagic Septicaemia HS is a major disease of cattle and buffaloes characterised by an acute highly fatal septicaemia with high morbidity and mortality. In Asia HS is caused by Pasteurella multocida serotype B:2 or 6:B. The production of iron regulated outer membrane proteins IROMPs is regulated by Fur protein through repression of the expression of the respective genes when complexed with iron. In this study outer membrane proteins OMPs and iron-regulated outer membrane proteins IROMPs were prepared by extraction with 1.5 sarcosyl from the strain cultured on BHI medium and on a medium supplemented with different concentration of 2 2;-dipyridyl. Outer membrane proteins from a Pasteurella multocida 6:B local isolate were characterised by using sodium dodecyl sulphate polyacrylamide gel electrophoresis SDS-PAGE technique. The approximate size of each OMPs detected were determined from log of molecular weight and Rf value. A total of 12 polypeptides ranging from 19.2 to 117.4 kDa were observed including 33 and 37kDa major OMPs of Pasteurella multocida and two other 117.4 and 91 kDa prominent protein bands.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Cattle |
| AGROVOC Term: | Pasteurella multocida |
| AGROVOC Term: | Haemorrhagic septicaemia |
| AGROVOC Term: | Bacterial diseases |
| AGROVOC Term: | Animal diseases |
| AGROVOC Term: | Serotypes |
| AGROVOC Term: | Genes |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Experiments |
| AGROVOC Term: | Polyacrylamide gel electrophoresis |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12969 |
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