Citation
Hwang Siaw San, . and Eng Lily, . and Poili Evenni, . (2011) Papaya dieback in Tawau in Sabah Malaysia. [Proceedings Paper]
Abstract
Papaya dieback was reported from Quoin Hill Station in Tawau in the Malaysian state of Sabah on the island of Borneo. The symptoms on the infected trees appeared to be similar to those reported in Peninsular Malaysia. Many papaya trees in Peninsular Malaysia have been destroyed by dieback. The causal organisms in West Malaysia have been identified as the bacteria Erwinia papayae and Erwinia mallotivora by two different groups of researchers. Isolation of the bacteria from infected papaya trees with dieback symptoms at Quoin Hill Station was carried out. Koch;s postulates were performed on all isolates. All five isolates produced infections with symptoms similar to those from the original infected hosts. The bacteria were re-isolated and cultured. DNA extractions were carried out and polymerase chain reactions using universal 16S rDNA primers were performed. Gel clean-up was done and sequencing carried out to identify the bacterial species. Sequencing data showed that the bacteria from Quoin Hill were more closely related to Erwinia mallotivora than to Erwinia papayae.
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Abstract
Papaya dieback was reported from Quoin Hill Station in Tawau in the Malaysian state of Sabah on the island of Borneo. The symptoms on the infected trees appeared to be similar to those reported in Peninsular Malaysia. Many papaya trees in Peninsular Malaysia have been destroyed by dieback. The causal organisms in West Malaysia have been identified as the bacteria Erwinia papayae and Erwinia mallotivora by two different groups of researchers. Isolation of the bacteria from infected papaya trees with dieback symptoms at Quoin Hill Station was carried out. Koch;s postulates were performed on all isolates. All five isolates produced infections with symptoms similar to those from the original infected hosts. The bacteria were re-isolated and cultured. DNA extractions were carried out and polymerase chain reactions using universal 16S rDNA primers were performed. Gel clean-up was done and sequencing carried out to identify the bacterial species. Sequencing data showed that the bacteria from Quoin Hill were more closely related to Erwinia mallotivora than to Erwinia papayae.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Carica |
| AGROVOC Term: | Carica papaya |
| AGROVOC Term: | Pawpaw carica papaya |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Disease symptoms |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | DNA sequence |
| AGROVOC Term: | Gel electrophoresis |
| AGROVOC Term: | Pathogens |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12973 |
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