Assessment of genetic diversity of Fusarium solani isolates on black pepper by inter-simple sequence repeat polymerase chain reaction


Citation

Khairulmazmi Ahmad, . and Sariah Meon, . and Shabanimofrad Mahmood Reza, . and Sahar Shahnazi, . and Ganesan Vadamalai, . (2011) Assessment of genetic diversity of Fusarium solani isolates on black pepper by inter-simple sequence repeat polymerase chain reaction. [Proceedings Paper]

Abstract

34 isolates of Fusarium solani were collected from major pepper growing areas in Sarawak and Johor. Isolations were carried by plating root sections from vines showing symptoms of yellowing disease on Fusarium media. Genomic DNA of Fusarium isolates was extracted using CTAB method. ISSR markers were amplified using 15 primers with di- or tri-nucleotide repeats in ISSR analysis. To study the genetic relationship a pairwise similarity matrix was generated using Jaccard;s similarity coefficient. Cluster analysis was performed to develop a dendrogram with NTSYS-pc using UPGMA. Based on the 15 primers fragment size obtained ranged from 200 pb to 3600 pb and a total of 253 amplified bands were generated. The percentage of polymorphism was 98. Dendrogram based on UPGMA clustering categorized the 34 F. solani isolates into two main clusters. Cluster 1 includes 33 isolates. This cluster was split into 2 sub cluster. Cluster 2 contained a unique isolate Fs-H6. This isolate showed a unique banding pattern and was distinct from other isolates. The values of similarity coefficients ranged from 0.145 for the lowest similarity coefficient between Fs-H6 and Fs-F2 to 0.848 for the greatest extent of similarity between Fs-K3 and Fs-H7. This work provided more information on the molecular evolution of F. solani isolates which are essential for black pepper breeding program against the yellowing disease.


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Abstract

34 isolates of Fusarium solani were collected from major pepper growing areas in Sarawak and Johor. Isolations were carried by plating root sections from vines showing symptoms of yellowing disease on Fusarium media. Genomic DNA of Fusarium isolates was extracted using CTAB method. ISSR markers were amplified using 15 primers with di- or tri-nucleotide repeats in ISSR analysis. To study the genetic relationship a pairwise similarity matrix was generated using Jaccard;s similarity coefficient. Cluster analysis was performed to develop a dendrogram with NTSYS-pc using UPGMA. Based on the 15 primers fragment size obtained ranged from 200 pb to 3600 pb and a total of 253 amplified bands were generated. The percentage of polymorphism was 98. Dendrogram based on UPGMA clustering categorized the 34 F. solani isolates into two main clusters. Cluster 1 includes 33 isolates. This cluster was split into 2 sub cluster. Cluster 2 contained a unique isolate Fs-H6. This isolate showed a unique banding pattern and was distinct from other isolates. The values of similarity coefficients ranged from 0.145 for the lowest similarity coefficient between Fs-H6 and Fs-F2 to 0.848 for the greatest extent of similarity between Fs-K3 and Fs-H7. This work provided more information on the molecular evolution of F. solani isolates which are essential for black pepper breeding program against the yellowing disease.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number
AGROVOC Term: Piper nigrum
AGROVOC Term: Piperaceae
AGROVOC Term: Black pepper
AGROVOC Term: Fusarium solani
AGROVOC Term: Fungal diseases
AGROVOC Term: Genetic diversity within species
AGROVOC Term: Disease symptoms
AGROVOC Term: Isolation
AGROVOC Term: Identification
AGROVOC Term: Electrophoresis
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/12981

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