Citation
Kairul Hafizi M. S., . and Siti Fatimah B. S., . and Mariana Nor Shamsudin, . and Abdelhadi Vasser Mohamed, . (2011) Antibiotic resistance and herbal sensitivity of Aeromonas and Pseudomonas bacteria isolated from cultured fish in the Egyptian and Malaysian environments. [Proceedings Paper]
Abstract
The sensitivity of certain commercial antibiotics and common herbs was evaluated against pathogenic Aeromonas hydrophila Aeromonas sobria Aeromonas caviae Pseudomonas fluorescens Pseudomonas putida and Pseudomonas aeruginosa isolated from Malaysian and Egyptian cultured fish mainly tilapia. A suspension of freshly cultured isolates was prepared with 0.5 OD and 100 l of this suspension was spread over the Muller;s Hinton agar plates. The antibiotic discs were inoculated on each cultured plate while the herbal extracts were soaked on Whatman filter paper 20 l each that have been cut into discs and later inserted on to bacteriacultured plates to screen their sensitivity to both antibiotics and herbs. Double-fold dilution was used to determine the minimal inhibitory concentration MIC for the effective herbs at 100 0.5 0.25 0.125 and 6.25. Results revealed high resistance of the tested bacteria against most of the screened antibiotics except Ciprofloxacin and Gentamycin. With regard to herbal sensitivity only Origanum vulgare showed effectiveness and inhibition zone against all isolates except A. hydrophila isolated from cultured tilapia in Egypt which exhibited resistance to that herb. The MIC ranged from 25-50 for both Egyptian and Malaysian isolates. Thus Origanum vulgare is recommended as a feed additive for cultured fish and can also be used with concentrations slightly less than the MIC for live-attenuated vaccines; preparation.
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Abstract
The sensitivity of certain commercial antibiotics and common herbs was evaluated against pathogenic Aeromonas hydrophila Aeromonas sobria Aeromonas caviae Pseudomonas fluorescens Pseudomonas putida and Pseudomonas aeruginosa isolated from Malaysian and Egyptian cultured fish mainly tilapia. A suspension of freshly cultured isolates was prepared with 0.5 OD and 100 l of this suspension was spread over the Muller;s Hinton agar plates. The antibiotic discs were inoculated on each cultured plate while the herbal extracts were soaked on Whatman filter paper 20 l each that have been cut into discs and later inserted on to bacteriacultured plates to screen their sensitivity to both antibiotics and herbs. Double-fold dilution was used to determine the minimal inhibitory concentration MIC for the effective herbs at 100 0.5 0.25 0.125 and 6.25. Results revealed high resistance of the tested bacteria against most of the screened antibiotics except Ciprofloxacin and Gentamycin. With regard to herbal sensitivity only Origanum vulgare showed effectiveness and inhibition zone against all isolates except A. hydrophila isolated from cultured tilapia in Egypt which exhibited resistance to that herb. The MIC ranged from 25-50 for both Egyptian and Malaysian isolates. Thus Origanum vulgare is recommended as a feed additive for cultured fish and can also be used with concentrations slightly less than the MIC for live-attenuated vaccines; preparation.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Antibiotic resistance |
| AGROVOC Term: | Aeromonas |
| AGROVOC Term: | Aeromonas hydrophila |
| AGROVOC Term: | Pseudomonas aeruginosa |
| AGROVOC Term: | Aquatic animals |
| AGROVOC Term: | Tilapia |
| AGROVOC Term: | Fish culture |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Culture media |
| AGROVOC Term: | Gentamycin |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12982 |
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