Citation
Kuan Khing Boon, . and Zulkifli H. Shamsuddin, . (2011) Isolation characterisation and selection of plant growth promoting rhizobacteria PGPR from maize roots. [Proceedings Paper]
Abstract
Plant Growth Promoting Rhizbacteria PGPR have very important roles as a biofertilizer in sustainable agriculture system. A total of 53 endophytic and rhizospheric isolates were isolated from maize roots at UPM Serdang Selangor and Sik Kedah. Soil pH of the sampling sites ranges from slightly acidic pH 5.2 to near neutral pH 6.8. Cultural characteristics determined were Gram stain bacterial shape and colony morphology. Nitrogen-fixing and phosphate solubilising abilities were qualitatively determined by N-free semi solid malate media and Pikovskaya media respectively while indole-3-acetic acid auxin producing ability was quantitatively determined using a spectrophotometer 535 nm. There were no specific influences of Gram stain soil pH bacterial shape or colony morphology on the plant growth promoting PGP abilities of the isolates. The location of sampling site influences the total number of isolates with PGP abilities. A rhizospheric isolate Fr2 produced the highest amount of auxin and abilities to fix Nand solubilise phosphate. Fr2 and 10 other more superior isolates will be selected for subsequent experiment on growth of maize plants in a growth chamber trial. This experiment provides an efficient rapid and cost effective method to screen potential PGPR isolates for maize plants before further selection via growth chamber pot and field trials.
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Abstract
Plant Growth Promoting Rhizbacteria PGPR have very important roles as a biofertilizer in sustainable agriculture system. A total of 53 endophytic and rhizospheric isolates were isolated from maize roots at UPM Serdang Selangor and Sik Kedah. Soil pH of the sampling sites ranges from slightly acidic pH 5.2 to near neutral pH 6.8. Cultural characteristics determined were Gram stain bacterial shape and colony morphology. Nitrogen-fixing and phosphate solubilising abilities were qualitatively determined by N-free semi solid malate media and Pikovskaya media respectively while indole-3-acetic acid auxin producing ability was quantitatively determined using a spectrophotometer 535 nm. There were no specific influences of Gram stain soil pH bacterial shape or colony morphology on the plant growth promoting PGP abilities of the isolates. The location of sampling site influences the total number of isolates with PGP abilities. A rhizospheric isolate Fr2 produced the highest amount of auxin and abilities to fix Nand solubilise phosphate. Fr2 and 10 other more superior isolates will be selected for subsequent experiment on growth of maize plants in a growth chamber trial. This experiment provides an efficient rapid and cost effective method to screen potential PGPR isolates for maize plants before further selection via growth chamber pot and field trials.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number |
| AGROVOC Term: | Zea mays |
| AGROVOC Term: | Poaceae |
| AGROVOC Term: | Maize |
| AGROVOC Term: | Plant growthpromoting rhizobacteria |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Absorbance |
| AGROVOC Term: | Soil ph |
| AGROVOC Term: | Indoleacetic acid |
| AGROVOC Term: | Phosphates |
| AGROVOC Term: | Nitrogen |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12985 |
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