Citation
Lau Wei Hong, . and Kong Lih Ling, . and Ganesan Vadamalai, . and Sathis Sri Thanarajoo, . and Jugah Kadir, . (2011) Development of loop-mediated isothermal amplification LAMP method for rapid detection of Coconut cadang-cadang viroid CCCVd variants in oil palm. [Proceedings Paper]
Abstract
Coconut cadang-cadang viroid CCCVd is the causal agent of the lethal Coconut cadang-cadang disease in the Philippines. CCCVd is also associated with orange spotting disease in oil palm. In 2006 Vadamalai and others have demonstrated that oil palms in Malaysia contained several sequence variants of CCCVd. These oil palm CCCVd variants were found in low concentration thus difficult to detect. Rapid detection of CCCVd is vital for prevention and control measures of OS. There are many methods for detecting CCCVd such as Polyacrylamide gel electrophoresis PAGE Ribonuclease protection Assay RPA RT-PCR and hybridization but they are time consuming and inconsistent for CCCVd oil palm variants. Therefore Reverse Transcription Loop-mediated Isothermal Amplification RT-LAMP a novel gene amplification method was evaluated for detection of oil palm CCCVd variants. CCCVd was detected within 60 min by the RT-LAMP method using total nucleic acid extracted from oil palm leaves. This method was able to detect the presence of CCCVd in the infected leaf samples where fluorescence reagent showed colour change from orange to green. Analysis of RT-LAMP product using 2 agarose gel electrophoresis also showed bands of expected size ca 150 bp thus confirming the presence of CCCVd in the oil palm samples.
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Abstract
Coconut cadang-cadang viroid CCCVd is the causal agent of the lethal Coconut cadang-cadang disease in the Philippines. CCCVd is also associated with orange spotting disease in oil palm. In 2006 Vadamalai and others have demonstrated that oil palms in Malaysia contained several sequence variants of CCCVd. These oil palm CCCVd variants were found in low concentration thus difficult to detect. Rapid detection of CCCVd is vital for prevention and control measures of OS. There are many methods for detecting CCCVd such as Polyacrylamide gel electrophoresis PAGE Ribonuclease protection Assay RPA RT-PCR and hybridization but they are time consuming and inconsistent for CCCVd oil palm variants. Therefore Reverse Transcription Loop-mediated Isothermal Amplification RT-LAMP a novel gene amplification method was evaluated for detection of oil palm CCCVd variants. CCCVd was detected within 60 min by the RT-LAMP method using total nucleic acid extracted from oil palm leaves. This method was able to detect the presence of CCCVd in the infected leaf samples where fluorescence reagent showed colour change from orange to green. Analysis of RT-LAMP product using 2 agarose gel electrophoresis also showed bands of expected size ca 150 bp thus confirming the presence of CCCVd in the oil palm samples.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number |
| AGROVOC Term: | Coconut cadang cadang |
| AGROVOC Term: | Viroids |
| AGROVOC Term: | Elaeis guineensis |
| AGROVOC Term: | Oil palms |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | RNA |
| AGROVOC Term: | Nucleic acids |
| AGROVOC Term: | Methodology |
| AGROVOC Term: | Gel electrophoresis |
| AGROVOC Term: | Turbidity |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12987 |
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