Citation
Ebrahimi Reza Farzin, . and Rosna Mat Taha, . (2011) A study on in vitro callus formation and biological activities of Gardenia jasminoides ellis. [Proceedings Paper]
Abstract
Leaf explants of Gardenia jasminoides Ellis were cultured on MS and WPM media supplemented with various concentrations of NAA IBA TDZ and Kn. After six months concentrations of 2 2.5 and 3 mg/L NAA and 2.5 and 3 mg/L of IBA showed 100 of callus formation on MS media. Hundred percentages of callus formation were observed On WPM media supplemented with 2 2.5 and 3 mg/L of IBA and the same concentrations of NAA. Higher percentages of callus 84 and 69 were observed on MS media supplemented with 1 mg/L Kinetin and TDZ respectively and WPM when supplemented with Kn 1 mg/L and TDZ 2 mg/L showed 58 and 25 of callus formation. In vitro extracts grown on WPM and MS media supplemented with NAA showed inhibition zone in antibacterial essay against Escherichia coli and Bacillus cereus. In vitro antioxidant activity showed significant differences compared to in vivo 20 extracts.
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Abstract
Leaf explants of Gardenia jasminoides Ellis were cultured on MS and WPM media supplemented with various concentrations of NAA IBA TDZ and Kn. After six months concentrations of 2 2.5 and 3 mg/L NAA and 2.5 and 3 mg/L of IBA showed 100 of callus formation on MS media. Hundred percentages of callus formation were observed On WPM media supplemented with 2 2.5 and 3 mg/L of IBA and the same concentrations of NAA. Higher percentages of callus 84 and 69 were observed on MS media supplemented with 1 mg/L Kinetin and TDZ respectively and WPM when supplemented with Kn 1 mg/L and TDZ 2 mg/L showed 58 and 25 of callus formation. In vitro extracts grown on WPM and MS media supplemented with NAA showed inhibition zone in antibacterial essay against Escherichia coli and Bacillus cereus. In vitro antioxidant activity showed significant differences compared to in vivo 20 extracts.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Gardenia |
| AGROVOC Term: | Rubiaceae |
| AGROVOC Term: | Callus |
| AGROVOC Term: | Explants |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | In vivo experimentation |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | Naphthaleneacetic acid |
| AGROVOC Term: | IBA |
| AGROVOC Term: | Extracts |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12998 |
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