Citation
Siti Nursyazana Md. Salleh, . and Ainon Hamzah, . and Sukiman Sarmani, . (2011) Effect of the inoculum size of microbial consortium in degrading various concentrations of tapis crude oil in soil. [Proceedings Paper]
Abstract
The objectives of this study were to optimize the size of inoculum of the microbial consortium and concentrations of Tapis crude oil for effective biodegradation. The soil without any history of hydrocarbon contamination was spiked with 5 w/w crude oil and added with various concentrations of microbial consortium. The size of microbial consortium was varied from 10 to 30 v/w. Degradation of crude oil was expressed as Total Petroleum Hydrocarbon TPH analyzed by gas chromatography with flame ionization detector GC-FID. The result showed the highest degradation of Tapis crude oil was at 97.8 of TPH was achieved when using 20 v/w of microbial consortium with 5 w/w of crude oil at day 10 of incubation. Further to optimize the concentration of crude oil it was varied from 10 to 20 w/w. For optimum concentration of crude oil with 20 v/w of microbial consortium produced the highest degradation when 10 w/w crude oil applied to the soil. The TPH degradation achieved was 100 at day 30 of incubation meanwhile the control plot which contained only indigenous microbes showed 30 of TPH degradation at day 30 of incubation.
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Abstract
The objectives of this study were to optimize the size of inoculum of the microbial consortium and concentrations of Tapis crude oil for effective biodegradation. The soil without any history of hydrocarbon contamination was spiked with 5 w/w crude oil and added with various concentrations of microbial consortium. The size of microbial consortium was varied from 10 to 30 v/w. Degradation of crude oil was expressed as Total Petroleum Hydrocarbon TPH analyzed by gas chromatography with flame ionization detector GC-FID. The result showed the highest degradation of Tapis crude oil was at 97.8 of TPH was achieved when using 20 v/w of microbial consortium with 5 w/w of crude oil at day 10 of incubation. Further to optimize the concentration of crude oil it was varied from 10 to 20 w/w. For optimum concentration of crude oil with 20 v/w of microbial consortium produced the highest degradation when 10 w/w crude oil applied to the soil. The TPH degradation achieved was 100 at day 30 of incubation meanwhile the control plot which contained only indigenous microbes showed 30 of TPH degradation at day 30 of incubation.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Crude oil |
| AGROVOC Term: | Biodegradation |
| AGROVOC Term: | Bioremediation |
| AGROVOC Term: | Pseudomonas |
| AGROVOC Term: | Acinetobacter |
| AGROVOC Term: | Trichoderma |
| AGROVOC Term: | Degradation |
| AGROVOC Term: | Soil ph |
| AGROVOC Term: | Soil moisture |
| AGROVOC Term: | Distilled water |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13005 |
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