Citation
Ummul Khair Mohd Syahriansva, . and Ainon Hamzah, . and Salmijah Surit, . (2011) Screening and identification of potential biosurfactant producer from hydrocarbon-degrading bacteria. [Proceedings Paper]
Abstract
The aim of this study was to screen potential biosurfactant producers from local isolates that are capable of degrading hydrocarbon. Screening of biosurfactant production was carried out by growing the isolates in minimal salt medium added with 0.1 v/v trace elements and 1 v/v sunflower oil as a sole carbon source. Production of biosurfactant was determined by surface tension measurement emulsification index E‚‚‚„ and oil spreading technique. Out of 25 bacterial isolates screened one potential biosurfactant producer was identified as Bacillus subtilis 4M5 based on morphological biochemical tests and 16S rRNA sequence analysis. This isolate reduced the surface tension of water from 72 mN/m to 41.75 mN/m with emulsification index E‚‚‚„ of 16 with crude oil.
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Abstract
The aim of this study was to screen potential biosurfactant producers from local isolates that are capable of degrading hydrocarbon. Screening of biosurfactant production was carried out by growing the isolates in minimal salt medium added with 0.1 v/v trace elements and 1 v/v sunflower oil as a sole carbon source. Production of biosurfactant was determined by surface tension measurement emulsification index E‚‚‚„ and oil spreading technique. Out of 25 bacterial isolates screened one potential biosurfactant producer was identified as Bacillus subtilis 4M5 based on morphological biochemical tests and 16S rRNA sequence analysis. This isolate reduced the surface tension of water from 72 mN/m to 41.75 mN/m with emulsification index E‚‚‚„ of 16 with crude oil.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Hydrocarbons |
| AGROVOC Term: | Degradation |
| AGROVOC Term: | Bioremediation |
| AGROVOC Term: | Screening tests |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Sunflower oil |
| AGROVOC Term: | Trace elements |
| AGROVOC Term: | Microorganisms |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13007 |
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