Citation
Ng Shee Ping, . and Palombo Enzo A., . and Bhave Mrina, . (2011) The heavy metal tolerant soil bacterium Achromobacter sp. strain AO22 encodes acop locus and its copper biosensor ability. [Proceedings Paper]
Abstract
Copper-containing compounds are introduced into the environment through agricultural products mining and metal industries and cause severe detrimental effects on ecosystems. Certain microorganisms exposed to these stressors appear to have developed mechanisms to maintain intracellular copper homeostasis and avoid toxicity. This study aimed to identify the copper homeostasis determinants from a multi-metal tolerant soil bacterium Achromobacter sp. A022. A cluster of genes designated copSRABGOFCDK was identified of which copSRAB and copCD are related to the cop operons of Pseudomonas syringae and Cupriavidus metallidurans CH34 and pco of E. coli which encode systems that sequester in or export excess copper from the periplasm. copF encodes a putative heavy metal P-type ATPase with a role of cytoplasmic copper efflux while copG copO and copK encode putative periplasmic copper chaperones. Sequencing of the flanking regions of the A022 cop locus indicated that it could be present on a genomic island GI. A section of DNA between copR and copA was functionally identified as a copper responsive promoter using a lacZ reporter construct pCOPRP in E. coli. In an engineered strain of A022 carrying the same plasmid designated A022 pCOPRP expression of lacZ was enhanced 140-fold in the presence of Cu but minimal when exposed to cadmium lead mercury. silver and zinc. This soil bacterium and its cop operon thus make excellent systems for studying copper homeostasis and designing biosensors for environmental copper contamination rather than the enteric E. coli.
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Abstract
Copper-containing compounds are introduced into the environment through agricultural products mining and metal industries and cause severe detrimental effects on ecosystems. Certain microorganisms exposed to these stressors appear to have developed mechanisms to maintain intracellular copper homeostasis and avoid toxicity. This study aimed to identify the copper homeostasis determinants from a multi-metal tolerant soil bacterium Achromobacter sp. A022. A cluster of genes designated copSRABGOFCDK was identified of which copSRAB and copCD are related to the cop operons of Pseudomonas syringae and Cupriavidus metallidurans CH34 and pco of E. coli which encode systems that sequester in or export excess copper from the periplasm. copF encodes a putative heavy metal P-type ATPase with a role of cytoplasmic copper efflux while copG copO and copK encode putative periplasmic copper chaperones. Sequencing of the flanking regions of the A022 cop locus indicated that it could be present on a genomic island GI. A section of DNA between copR and copA was functionally identified as a copper responsive promoter using a lacZ reporter construct pCOPRP in E. coli. In an engineered strain of A022 carrying the same plasmid designated A022 pCOPRP expression of lacZ was enhanced 140-fold in the presence of Cu but minimal when exposed to cadmium lead mercury. silver and zinc. This soil bacterium and its cop operon thus make excellent systems for studying copper homeostasis and designing biosensors for environmental copper contamination rather than the enteric E. coli.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Copper |
| AGROVOC Term: | Heavy metals |
| AGROVOC Term: | Ecosystems |
| AGROVOC Term: | Homeostasis |
| AGROVOC Term: | Metals |
| AGROVOC Term: | Tolerance |
| AGROVOC Term: | Cloning |
| AGROVOC Term: | Enzyme activity |
| AGROVOC Term: | Enzymes |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13010 |
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