Isolation screening and identification of newly isolated acrylamide-degrading bacterium

Norzila Kusnin, . and Siti Aqlima Ahmad, . and Mohd. Yunus Abdul Shukor, . and Mohd. Arif Syed, . (2011) Isolation screening and identification of newly isolated acrylamide-degrading bacterium. [Proceedings Paper]

Acrylamide is a toxic and carcinogenic compound which causes irritation to the skin and respiratory tract and may also cause cancer. The main source of acrylamide pollution is the release of monomer residues from polyacrylamide which is widely used for water treatment. Previous studies have shown that bacteria isolated from palm-oil plantations soil that are contaminated with pesticides possess the amidase enzyme needed to degrade acrylamide to acrylic acid and ammonia. In this study bacteria isolated from 40 soil samples and 10 water samples from various locations in Malaysia were screened for acrylamide-degrading ability. The best bacterial isolate was selected based on the ability to degrade high concentrations of acrylamide 0.6 g/L at the highest rate and was identified using 16S rRNA. This bacterium was grown in basal salt media using acrylamide as the sole nitrogen source for growth. The degradation of acrylamide was then qualitatively analysed using high performance liquid chromatography HPLC with UV spectrophotometric detection at 196 nm.

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Abstract

Acrylamide is a toxic and carcinogenic compound which causes irritation to the skin and respiratory tract and may also cause cancer. The main source of acrylamide pollution is the release of monomer residues from polyacrylamide which is widely used for water treatment. Previous studies have shown that bacteria isolated from palm-oil plantations soil that are contaminated with pesticides possess the amidase enzyme needed to degrade acrylamide to acrylic acid and ammonia. In this study bacteria isolated from 40 soil samples and 10 water samples from various locations in Malaysia were screened for acrylamide-degrading ability. The best bacterial isolate was selected based on the ability to degrade high concentrations of acrylamide 0.6 g/L at the highest rate and was identified using 16S rRNA. This bacterium was grown in basal salt media using acrylamide as the sole nitrogen source for growth. The degradation of acrylamide was then qualitatively analysed using high performance liquid chromatography HPLC with UV spectrophotometric detection at 196 nm.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Bacteria
AGROVOC Term:	Acrylamide
AGROVOC Term:	Degradation
AGROVOC Term:	Isolation
AGROVOC Term:	Identification
AGROVOC Term:	Screening tests
AGROVOC Term:	Pollution
AGROVOC Term:	Soil sampling
AGROVOC Term:	Mineral salts nutrients
AGROVOC Term:	Trace elements
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13011

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