Isolation and identification of oil degrading microorganisms from oily wastewater

Marina Bujang, . and Aweng Eh Rak, . and Noor Azlina Ibrahim, . (2011) Isolation and identification of oil degrading microorganisms from oily wastewater. [Proceedings Paper]

Oil degrading bacteria were isolated from oily wastewater collected from automotive workshop in Kota Bharu. Isolation was done by using enrichment media using diesel as the carbon and energy sources. The isolates were identified based on morphology including gram staining and molecular properties. Four isolates that showed higher growth during screening as demonstrated by increase in their optical density OD‚‚‚ were selected for further studies. Molecular identification of the isolates based on 16S rDNA gene sequences assigned them as Bacillus cereus Bacillus anthracis Acinetobacter iunni and Staphylococcus. The ability of Bacillus cereus Bacillus anthracis Acinetobacter iunni and Staphylococcus to grow in the presence c: increasing concentration of hydrocarbon was studied. It was found that Bacillus cereus exhibited higher growth among the isolates and was used for further studies. Bacillus anthracis Acinetobacter iunni and Staphylococcus were not subjected to degradation test because these bacteria did not grow well in the presence of increasing hydrocarbon concentration. Biodegradation activity was tested by using liquid culture characteristic and emulsification activity. In both tests the results show maxima increase in optical densities and emulsification activity concomitant with decrease in pH of the culture media. These findings suggest that isolate KA can be applied for bioremediation of hydrocarbon containing oily wastewater.

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Abstract

Oil degrading bacteria were isolated from oily wastewater collected from automotive workshop in Kota Bharu. Isolation was done by using enrichment media using diesel as the carbon and energy sources. The isolates were identified based on morphology including gram staining and molecular properties. Four isolates that showed higher growth during screening as demonstrated by increase in their optical density OD‚‚‚ were selected for further studies. Molecular identification of the isolates based on 16S rDNA gene sequences assigned them as Bacillus cereus Bacillus anthracis Acinetobacter iunni and Staphylococcus. The ability of Bacillus cereus Bacillus anthracis Acinetobacter iunni and Staphylococcus to grow in the presence c: increasing concentration of hydrocarbon was studied. It was found that Bacillus cereus exhibited higher growth among the isolates and was used for further studies. Bacillus anthracis Acinetobacter iunni and Staphylococcus were not subjected to degradation test because these bacteria did not grow well in the presence of increasing hydrocarbon concentration. Biodegradation activity was tested by using liquid culture characteristic and emulsification activity. In both tests the results show maxima increase in optical densities and emulsification activity concomitant with decrease in pH of the culture media. These findings suggest that isolate KA can be applied for bioremediation of hydrocarbon containing oily wastewater.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Microorganisms
AGROVOC Term:	Biodegradation
AGROVOC Term:	Degradation
AGROVOC Term:	Energy sources
AGROVOC Term:	Microbial degradation
AGROVOC Term:	Isolation
AGROVOC Term:	Identification
AGROVOC Term:	Cultivation
AGROVOC Term:	Screening tests
AGROVOC Term:	Carbon
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13021

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