Citation
Azham Zulkharnain, . and Taka Jane Sebestian, . (2011) Preliminary studies on heterocyclic hydrocarbon degrading bacteria isolated from Southwest coast of Borneo. [Proceedings Paper]
Abstract
Isolation of heterocyclic hydrocarbon degrading bacteria from seawater samples collected from Pulau Satang Muara Tebas Sematan and Miri. Carbazole biphenyl dibenzofuran and dibenzothiophene were used as sole carbon sources during enrichment growth with artificial seawater medium ONR7a. A total of six isolates were successfully isolated. Accumulation of metabolites through appearance of colors in media was confirmed. Isolate designated MT01 and MT02 showed positive growth using biphenyl and dibenzothiophene as sole carbon source while isolates M01 M02 and M03 showed positive growth using biphenyl dibenzothiophene and carbazole. However isolate PS01 from Pulau Satang could only grow on biphenyl. All six isolates were characterized genetically by 16S ribosomal DNA sequence where PS01; MT01 and MT02; and M01 and M02 were identified as Thalassospira xiamenensis Thalassospira xianheensis and Thalassospira profundimaris respectively. Isolate M03 was identified as Pseudomonas pachasfrellae.
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Abstract
Isolation of heterocyclic hydrocarbon degrading bacteria from seawater samples collected from Pulau Satang Muara Tebas Sematan and Miri. Carbazole biphenyl dibenzofuran and dibenzothiophene were used as sole carbon sources during enrichment growth with artificial seawater medium ONR7a. A total of six isolates were successfully isolated. Accumulation of metabolites through appearance of colors in media was confirmed. Isolate designated MT01 and MT02 showed positive growth using biphenyl and dibenzothiophene as sole carbon source while isolates M01 M02 and M03 showed positive growth using biphenyl dibenzothiophene and carbazole. However isolate PS01 from Pulau Satang could only grow on biphenyl. All six isolates were characterized genetically by 16S ribosomal DNA sequence where PS01; MT01 and MT02; and M01 and M02 were identified as Thalassospira xiamenensis Thalassospira xianheensis and Thalassospira profundimaris respectively. Isolate M03 was identified as Pseudomonas pachasfrellae.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Heterocyclic compounds |
| AGROVOC Term: | Hydrocarbons |
| AGROVOC Term: | Degradation |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Sea water |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Carbon |
| AGROVOC Term: | Deoxyribonucleic acid |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Gel electrophoresis |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13022 |
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