Resazurin assay for determination of antimicrobial activity in Dillenia ovata

Lim Erin Swee Hua, . and Loh Tyi Yng, . and David Sheba, . and Pichika Mallikarjuna Rao, . and Oh Chai Ping, . and Ng Siew Ling, . and Poon Pui Mun, . and Chellian Jestin, . and Ng Shi Ying, . and Sin Xiao Jie, . (2011) Resazurin assay for determination of antimicrobial activity in Dillenia ovata. [Proceedings Paper]

This study was conducted to investigate the antimicrobial activities of Dillenia ovata in Gram positive and Gram negative bacteria fungi and yeasts. The resazurin cell viability assay was used in determining the Minimum Inhibitory Concentration MIC of the leaves and stem bark of D. ovata from which four extracts namely hexane methanol ethyl acetate and water were derived. The methanol extract from the stem bark were found to be most potent in all Gram negative bacteria tested by inhibiting growth at low concentrations of this extract. Interestingly fungi and yeasts were most sensitive to hexane extract obtained from the leaves and is non-susceptible to all other extracts. No inhibition of growth was observed for water extracts of both stem bark and leaves for all organisms. Resazurin viability assay is a simple effective method which enables the testing of samples in broth microdilutions with a clear-cut end point.

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Abstract

This study was conducted to investigate the antimicrobial activities of Dillenia ovata in Gram positive and Gram negative bacteria fungi and yeasts. The resazurin cell viability assay was used in determining the Minimum Inhibitory Concentration MIC of the leaves and stem bark of D. ovata from which four extracts namely hexane methanol ethyl acetate and water were derived. The methanol extract from the stem bark were found to be most potent in all Gram negative bacteria tested by inhibiting growth at low concentrations of this extract. Interestingly fungi and yeasts were most sensitive to hexane extract obtained from the leaves and is non-susceptible to all other extracts. No inhibition of growth was observed for water extracts of both stem bark and leaves for all organisms. Resazurin viability assay is a simple effective method which enables the testing of samples in broth microdilutions with a clear-cut end point.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Dilleniaceae
AGROVOC Term:	Antimicrobials
AGROVOC Term:	Antimicrobial properties
AGROVOC Term:	Leaves
AGROVOC Term:	Bark
AGROVOC Term:	Extracts
AGROVOC Term:	Extraction
AGROVOC Term:	Bacteria
AGROVOC Term:	Yeasts
AGROVOC Term:	Fungi
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13029

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