Inhibition of quorum sensing regulated biofilm formation in Pseudomonas aeruginosa by Malaysian dietary plants

Vun-Sang Senty, . and Zarina Amin, . (2011) Inhibition of quorum sensing regulated biofilm formation in Pseudomonas aeruginosa by Malaysian dietary plants. [Proceedings Paper]

Antiquorum sensing AQS activity and the inhibitory action against Pseudomonas aeruginosa PAO1 QS regulated biofilm production were investigated on ten aqueous and ethanol dietary plant extracts. The AQS activity of plant extracts was screened using the Chromobacterium violaceum CV N-acyl homoserine lactone AHL bioassay while biofilm investigation was performed using the crystal violet assay. In this study the extracts tested did not show any AQS activity in the CV AHL bioassay. However all ten plants from aqueous extract exhibited reduction in biofilm formation by at least 50. In contrast to aqueous extracts ethanol extracts did not show substantial effects in the inhibition of PAO1 biofilm virulence production. The ability to interrupt virulence factor production as shown by several dietary plants species suggested the potential of exploring natural products with GRAS generally regarded as safe properties for the development of further antibacterial therapy.

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Abstract

Antiquorum sensing AQS activity and the inhibitory action against Pseudomonas aeruginosa PAO1 QS regulated biofilm production were investigated on ten aqueous and ethanol dietary plant extracts. The AQS activity of plant extracts was screened using the Chromobacterium violaceum CV N-acyl homoserine lactone AHL bioassay while biofilm investigation was performed using the crystal violet assay. In this study the extracts tested did not show any AQS activity in the CV AHL bioassay. However all ten plants from aqueous extract exhibited reduction in biofilm formation by at least 50. In contrast to aqueous extracts ethanol extracts did not show substantial effects in the inhibition of PAO1 biofilm virulence production. The ability to interrupt virulence factor production as shown by several dietary plants species suggested the potential of exploring natural products with GRAS generally regarded as safe properties for the development of further antibacterial therapy.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number
AGROVOC Term:	Pseudomonas
AGROVOC Term:	Pseudomonas aeruginosa
AGROVOC Term:	Antibacterial properties
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Homoserine
AGROVOC Term:	Vegetables
AGROVOC Term:	Extracts
AGROVOC Term:	Extraction
AGROVOC Term:	Screening tests
AGROVOC Term:	Inhibition
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13033

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