Citation
Jelin Sawei, . and Norrakiah Abdullah Sani, . and Aminah Abdullah, . and Sahilah Abd. Mutalib, . (2011) Validation of food-borne pathogen detection using DNA microarray technology. [Proceedings Paper]
Abstract
This study was conducted to validate the capability of DNA microarray technology of Olipro„ FoodPATH Gene Chip in detecting food-borne pathogens. Nine types of food-borne pathogen DNAs were used namely Bacillus cereus Campylobacter spp. Escherichia coli 0157:H7 Listeria monocytogenes Salmonella spp. Shigella spp. Staphylococcus aureus Vibrio cholerae and Vibrio parahaemolyticus. A total of 36 combinations of DNA templates of food-borne pathogen were used. The validation using this microarray technology involved polymerase chain reaction PCR followed Southern-Blotting Hybridisation analysis on the chip. The results using SouthernBlotting Hybridization analysis on the chip was compared with the result from gel electrophoresis. Five selections were needed to complete the 36 combinations of DNA templates for food-borne pathogen. One of the chips was used as negative control whereby no DNA template of food-borne pathogen was inoculated. Results showed that all combinations of food-borne pathogen DNA templates were detected. The negative control chip did not show the presence of any DNA. Thus from this study it shows that Olipro„ FoodPATH Gene Chip gave better results than the 2.0 w/v gel electrophoresis.
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Abstract
This study was conducted to validate the capability of DNA microarray technology of Olipro„ FoodPATH Gene Chip in detecting food-borne pathogens. Nine types of food-borne pathogen DNAs were used namely Bacillus cereus Campylobacter spp. Escherichia coli 0157:H7 Listeria monocytogenes Salmonella spp. Shigella spp. Staphylococcus aureus Vibrio cholerae and Vibrio parahaemolyticus. A total of 36 combinations of DNA templates of food-borne pathogen were used. The validation using this microarray technology involved polymerase chain reaction PCR followed Southern-Blotting Hybridisation analysis on the chip. The results using SouthernBlotting Hybridization analysis on the chip was compared with the result from gel electrophoresis. Five selections were needed to complete the 36 combinations of DNA templates for food-borne pathogen. One of the chips was used as negative control whereby no DNA template of food-borne pathogen was inoculated. Results showed that all combinations of food-borne pathogen DNA templates were detected. The negative control chip did not show the presence of any DNA. Thus from this study it shows that Olipro„ FoodPATH Gene Chip gave better results than the 2.0 w/v gel electrophoresis.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol. 1 Call Number. |
| AGROVOC Term: | Pathogens |
| AGROVOC Term: | Foodborne diseases |
| AGROVOC Term: | Screening tests |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Hybridization |
| AGROVOC Term: | Bacillus cereus |
| AGROVOC Term: | Campylobacter |
| AGROVOC Term: | Escherichia coli |
| AGROVOC Term: | Listeria monocytogenes |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13042 |
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