Citation
Nik Hafizah Nik Ubaidillah, . and Noorlidah Abdullah, . and Annuar M. S. M., . and Vikineswary Sabaratnam, . (2011) Optimisation of medium composition for submerged fermentation of Ganoderma lucidum Cendawan senduk using response surface analysis. [Proceedings Paper]
Abstract
The present study aims to optimize the carbon and nitrogen requirements for the formulation of a low cost medium for cultivation of a local Ganoderma lucidum Cendawan Senduk in submerged fermentation. The ingredients investigated for cultivation medium were brown sugar and brewery spent yeast. The response surface analysis was used to determine the optimal percentage of carbon and nitrogen in the medium that resulted in the highest mycelia biomass as a response. The model predicted the optimal carbon and nitrogen levels in combination as brown sugar and brewery spent yeast at 10 C and 0.075 N with the mycelial dry weight of 21.50 0.049 g/L. The mycelia biomass values from validation experiments agreed well with the predicted values. Conclusively the response surface analysis using central composite design CCD was determined the optimal medium for G. lucidum cendawan senduk cultivation which composed of 37.25 g/l brewery spent yeast 91.3 g/l brown sugar 0.5 g/l MgSO‚„ 7H20 0.5 g/l KH‚‚PO‚„ and 0.5 g/l K‚‚HPO‚„. The optimum condition of carbon and nitrogen obtained from the statistical design will be useful to develop G. lucidum cendawan senduk cultivation process for efficient production.
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Abstract
The present study aims to optimize the carbon and nitrogen requirements for the formulation of a low cost medium for cultivation of a local Ganoderma lucidum Cendawan Senduk in submerged fermentation. The ingredients investigated for cultivation medium were brown sugar and brewery spent yeast. The response surface analysis was used to determine the optimal percentage of carbon and nitrogen in the medium that resulted in the highest mycelia biomass as a response. The model predicted the optimal carbon and nitrogen levels in combination as brown sugar and brewery spent yeast at 10 C and 0.075 N with the mycelial dry weight of 21.50 0.049 g/L. The mycelia biomass values from validation experiments agreed well with the predicted values. Conclusively the response surface analysis using central composite design CCD was determined the optimal medium for G. lucidum cendawan senduk cultivation which composed of 37.25 g/l brewery spent yeast 91.3 g/l brown sugar 0.5 g/l MgSO‚„ 7H20 0.5 g/l KH‚‚PO‚„ and 0.5 g/l K‚‚HPO‚„. The optimum condition of carbon and nitrogen obtained from the statistical design will be useful to develop G. lucidum cendawan senduk cultivation process for efficient production.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol. 1 Call Number. |
| AGROVOC Term: | Ganoderma |
| AGROVOC Term: | Ganoderma lucidum |
| AGROVOC Term: | Tropical rain forests |
| AGROVOC Term: | Cultivation |
| AGROVOC Term: | Fermentation |
| AGROVOC Term: | Culture media |
| AGROVOC Term: | Carbon |
| AGROVOC Term: | Nitrogen |
| AGROVOC Term: | Growth rate |
| AGROVOC Term: | Yeasts |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13045 |
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