Comparison of different cultural protocols in isolation of Yersinia enterocolitica

Tan Lai Kuan, . and Ooi Peck Toung, . and Thong Kwai Lin, . (2011) Comparison of different cultural protocols in isolation of Yersinia enterocolitica. [Proceedings Paper]

Yersinia enterocolitica is the third most important foodborne enteric pathogen that causes gastroenteritis in Europe after campylobacteriosis and salmonellosis. There are numerous enrichment schemes available in isolating Y. enterocolitica. However the isolation and recovery of Y. enterocolitica is very contingent difficult and time consuming. The lack of good isolation methods mask and underestimate the actual incidence of yersiniosis. The main objective of this study was to compare the recovery ability of different enrichment schemes and plating methods in the isolation of Y. enterocolitica. Six cultural protocols all comprising of a combination of three enrichment broths namely Yersinia selective enrichment broth according to OSSMER YSEO irgasan-ticarcillin-potassium chlorate ITG and phosphate buffered saline PBS cefsulodin-irgasan-novobiocin GIN as selective plating agar with or without a post-enrichment alkaline treatment were evaluated. Gold enrichment at 4G for 3 weeks using PBS followed by a post enrichment alkaline treatment and plating on GIN agar was the best among the six protocols evaluated and the detection limit was 102 GFU/mL.

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Abstract

Yersinia enterocolitica is the third most important foodborne enteric pathogen that causes gastroenteritis in Europe after campylobacteriosis and salmonellosis. There are numerous enrichment schemes available in isolating Y. enterocolitica. However the isolation and recovery of Y. enterocolitica is very contingent difficult and time consuming. The lack of good isolation methods mask and underestimate the actual incidence of yersiniosis. The main objective of this study was to compare the recovery ability of different enrichment schemes and plating methods in the isolation of Y. enterocolitica. Six cultural protocols all comprising of a combination of three enrichment broths namely Yersinia selective enrichment broth according to OSSMER YSEO irgasan-ticarcillin-potassium chlorate ITG and phosphate buffered saline PBS cefsulodin-irgasan-novobiocin GIN as selective plating agar with or without a post-enrichment alkaline treatment were evaluated. Gold enrichment at 4G for 3 weeks using PBS followed by a post enrichment alkaline treatment and plating on GIN agar was the best among the six protocols evaluated and the detection limit was 102 GFU/mL.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol. 1 Call Number.
AGROVOC Term:	Yersinia
AGROVOC Term:	Yersinia enterocolitica
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Pathogens
AGROVOC Term:	Foodborne diseases
AGROVOC Term:	Human diseases
AGROVOC Term:	Isolation
AGROVOC Term:	Methodology
AGROVOC Term:	Culture techniques
AGROVOC Term:	Microflora
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13053

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