Citation
Muhammad Anas Othaman, . and Shaiful Adzni Sharifudin, . and Kamariah Long, . (2011) Isolation identification and characterisation of lactic acid bacteria from coconut waste in Malaysia. [Proceedings Paper]
Abstract
Lactic acid bacteria LAB were isolated from coconut waste samples that have been collected from several states in Malaysia. 139 isolates that grew on MRS agar were picked and coded. All the isolates were identified by using 16sRNA gene. Lactobacillus strains showed its domination among all isolates with 98 isolates out of 139 and L. plantarum is the most species occurred. All the LAB strains were subjected to the hemolytic test and 22 isolates showed „-hemolysis effect and the rest showed ´-hemolysis effect. There was no single strain that showed -hemolysis effect. The ability of selected LAB to grow in coconut based medium was put on the test. All the isolates showed good proliferation in coconut based medium with the final pH ranged between 5.57-3.69 after 24h incubation. While the CFU ranged between 7.17-9.82 log CFU/mL. From this study it was found that Lactobacillus species dominates in coconut waste and have excellent proliferation in coconut based medium.
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Abstract
Lactic acid bacteria LAB were isolated from coconut waste samples that have been collected from several states in Malaysia. 139 isolates that grew on MRS agar were picked and coded. All the isolates were identified by using 16sRNA gene. Lactobacillus strains showed its domination among all isolates with 98 isolates out of 139 and L. plantarum is the most species occurred. All the LAB strains were subjected to the hemolytic test and 22 isolates showed „-hemolysis effect and the rest showed ´-hemolysis effect. There was no single strain that showed -hemolysis effect. The ability of selected LAB to grow in coconut based medium was put on the test. All the isolates showed good proliferation in coconut based medium with the final pH ranged between 5.57-3.69 after 24h incubation. While the CFU ranged between 7.17-9.82 log CFU/mL. From this study it was found that Lactobacillus species dominates in coconut waste and have excellent proliferation in coconut based medium.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol. 1 Call Number. |
| AGROVOC Term: | Lactic acid bacteria |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Fermentation |
| AGROVOC Term: | Coconuts |
| AGROVOC Term: | Wastes |
| AGROVOC Term: | Gene sequence |
| AGROVOC Term: | Genes |
| AGROVOC Term: | Lactobacillus |
| AGROVOC Term: | Lactococcus |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13055 |
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