Citation
Kamarudin M. S., . and Saad C. R., . and Balcazar J. L., . and Hosseini S., . and Zokaei Far H., . and Hosseini S. A., . and Sijam K., . (2011) In vitro evaluation of probiotic properties of bacteria isolated from fermented pickle. [Proceedings Paper]
Abstract
In the present studytwo potential antagonistic bacteria Bacillus subtilis strains L10 and G1 isolated from fermented pickle were evaluated for their probiotic properties in vitro. Molecular identification using specific primers effects of dilution cell free supernatant on Vibrio harveyi diluted factor; 0 25 50 and 75 temperature stability -20 60 80 120C types of extracellular enzymes Protease Amylase and Cellulase and production of antibacterial component proteinase K trypsin lysozyme lipase and aamylase by candidate strains have been investigated. Diluted cell free supernatant of both strains showed a great inhibitory activity against V. harveyi even at the highest dilution factor 75. Poor heat stability of cell free supernatant of both strains was observed when the supernatants were heated at the mentioned temperatures for 20 min; however strong inhibitory activity of cell free supernatant of both strains against V. harveyi was discovered after 7 month of freezing in -20C. Considerable reduction of antibacterial activity was recorded when the cell free supernatant of both strains were treated by all the subjected enzymes. It can be pointed out that the productions of antimicrobial peptide or inhibitory enzymes for both strains play the role of antagonism activity against V. harveyi. In addition production of extracellular enzymes such as protease and amylase by both strains were assessed whereas both strains were not able to produce cellulase enzyme. In conclusion based on the result of this study both strains could be considered as good potential probiotics for shrimp aquaculture.
Download File
Full text available from:
|
Abstract
In the present studytwo potential antagonistic bacteria Bacillus subtilis strains L10 and G1 isolated from fermented pickle were evaluated for their probiotic properties in vitro. Molecular identification using specific primers effects of dilution cell free supernatant on Vibrio harveyi diluted factor; 0 25 50 and 75 temperature stability -20 60 80 120C types of extracellular enzymes Protease Amylase and Cellulase and production of antibacterial component proteinase K trypsin lysozyme lipase and aamylase by candidate strains have been investigated. Diluted cell free supernatant of both strains showed a great inhibitory activity against V. harveyi even at the highest dilution factor 75. Poor heat stability of cell free supernatant of both strains was observed when the supernatants were heated at the mentioned temperatures for 20 min; however strong inhibitory activity of cell free supernatant of both strains against V. harveyi was discovered after 7 month of freezing in -20C. Considerable reduction of antibacterial activity was recorded when the cell free supernatant of both strains were treated by all the subjected enzymes. It can be pointed out that the productions of antimicrobial peptide or inhibitory enzymes for both strains play the role of antagonism activity against V. harveyi. In addition production of extracellular enzymes such as protease and amylase by both strains were assessed whereas both strains were not able to produce cellulase enzyme. In conclusion based on the result of this study both strains could be considered as good potential probiotics for shrimp aquaculture.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol. 1 Call Number. |
| AGROVOC Term: | Bacillus subtilis |
| AGROVOC Term: | Antagonistic bacteria |
| AGROVOC Term: | Evaluation |
| AGROVOC Term: | In vitro |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Fermented foods |
| AGROVOC Term: | Pickles |
| AGROVOC Term: | Vibrio |
| AGROVOC Term: | Temperature |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13057 |
Actions (login required)
 |
View Item |
