Isolation and identification of Lactobaccilus with antimicrobial properties from a food supplement

Foo Yoke Wai, . and Philip Koshy, . and Sekaran Muniandy, . (2011) Isolation and identification of Lactobaccilus with antimicrobial properties from a food supplement. [Proceedings Paper]

Lactobacillus is a genus of bacteria that produces lactic acid as an end product and can be found in a variety of plants and animals. Lactobacillus is traditionally used as natural food preservatives as it has the ability to inhibit the growth of food pathogens. The objective of this study is to determine if the isolated lactobacilli from a food supplement has any inhibitory activity on food pathogens. Lactobacillus was isolated from a food supplement in the current study using De Man Rogosa and Sharpe MRS agar. The isolated lactobacillus were then identified using 16s rRNA molecular method. The identified Lactobacillus were then cultured in MRS broth and the crude peptide broth was harvested and tested against selected test microorganisms using well diffusion method to determine its antimicrobial effect. Both strains of Lactobacillus rhamnosus BR1 and BR4 showed inhibitory activity against E.coIi0157:H7 but only L. rhamnosus strain BR4 showed inhibition against E.coli UT189. Lactobacillus fermentum TB1 showed good inhibition against Staphylococcus aureus compared to L. rhamnosus.

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Abstract

Lactobacillus is a genus of bacteria that produces lactic acid as an end product and can be found in a variety of plants and animals. Lactobacillus is traditionally used as natural food preservatives as it has the ability to inhibit the growth of food pathogens. The objective of this study is to determine if the isolated lactobacilli from a food supplement has any inhibitory activity on food pathogens. Lactobacillus was isolated from a food supplement in the current study using De Man Rogosa and Sharpe MRS agar. The isolated lactobacillus were then identified using 16s rRNA molecular method. The identified Lactobacillus were then cultured in MRS broth and the crude peptide broth was harvested and tested against selected test microorganisms using well diffusion method to determine its antimicrobial effect. Both strains of Lactobacillus rhamnosus BR1 and BR4 showed inhibitory activity against E.coIi0157:H7 but only L. rhamnosus strain BR4 showed inhibition against E.coli UT189. Lactobacillus fermentum TB1 showed good inhibition against Staphylococcus aureus compared to L. rhamnosus.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol. 1 Call Number.
AGROVOC Term:	Lactobacillus
AGROVOC Term:	Antimicrobial properties
AGROVOC Term:	Isolation
AGROVOC Term:	Identification
AGROVOC Term:	Food supplements
AGROVOC Term:	Ribosomal rna
AGROVOC Term:	Electrophoresis
AGROVOC Term:	DNA sequence
AGROVOC Term:	Spectrophotometers
AGROVOC Term:	PCR
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13058

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