Citation
Ann Anni Basik, . and Cyntia Seta, . and Holed Juboi, . and Yeo Tiong Chia, . (2011) Genetic chemical and bioactivity characterisation of actinomycetes isolates from Sarawak. [Proceedings Paper]
Abstract
One thousand actinomycetes strains from a library of 8434 strains were randomly revived and identified based on their 16S rDNA sequences. Classification of these sequences showed that the 1000 strains consisted of 11 different Actinobacteria families. Most of the strains 736 in this study were grouped under the Streptomycetaceae family followed by Micromomosporaceae 2-Streptosporangiaceae 32 Nocardiaceae 15 Pseudonocardiaceae 2 Thermomonosporaceae 2 Bacillaceae 1 Dermacoccaceae 1 Actinomycetacea 1 Mycobacteriaceae 1 and unclassified Actinomycetales 1. Under the Micromomosporaceae family rare genera such as Micromonospora 154 ar: Dactylosporangium 35 were prevalent. After genotypic dereplication 475 groups with similar 16S rDNA sequences were generated. All of the 1000 strains extracts were also subjected for antimicrobial screening against four test strains which exhibited 45 bioactivity ranging from narrow to broad spectrum activity. To study the relationship between the strains; genetic bioactivity and chemical profile chemical profiling of representative strains with similar 16S rDNA sequences were conducted. Sampling locations to compare its chemical profile and bioactivity were also included. In this study it was found that strains with similar 16S rDNA sequences from similar location can exhibit varying chemical profiles and bioactivity. The varying chemical profiles in these groups with similar 16S rDNA sequences were associated to the strains differential bioactivity pattern. This thereby implies that there is greater phenotype diversity beyond 16S rDNA genotyping.
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Abstract
One thousand actinomycetes strains from a library of 8434 strains were randomly revived and identified based on their 16S rDNA sequences. Classification of these sequences showed that the 1000 strains consisted of 11 different Actinobacteria families. Most of the strains 736 in this study were grouped under the Streptomycetaceae family followed by Micromomosporaceae 2-Streptosporangiaceae 32 Nocardiaceae 15 Pseudonocardiaceae 2 Thermomonosporaceae 2 Bacillaceae 1 Dermacoccaceae 1 Actinomycetacea 1 Mycobacteriaceae 1 and unclassified Actinomycetales 1. Under the Micromomosporaceae family rare genera such as Micromonospora 154 ar: Dactylosporangium 35 were prevalent. After genotypic dereplication 475 groups with similar 16S rDNA sequences were generated. All of the 1000 strains extracts were also subjected for antimicrobial screening against four test strains which exhibited 45 bioactivity ranging from narrow to broad spectrum activity. To study the relationship between the strains; genetic bioactivity and chemical profile chemical profiling of representative strains with similar 16S rDNA sequences were conducted. Sampling locations to compare its chemical profile and bioactivity were also included. In this study it was found that strains with similar 16S rDNA sequences from similar location can exhibit varying chemical profiles and bioactivity. The varying chemical profiles in these groups with similar 16S rDNA sequences were associated to the strains differential bioactivity pattern. This thereby implies that there is greater phenotype diversity beyond 16S rDNA genotyping.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Actinomycetes |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Classification |
| AGROVOC Term: | Genetics |
| AGROVOC Term: | DNA sequence |
| AGROVOC Term: | Bacillaceae |
| AGROVOC Term: | Actinomycetales |
| AGROVOC Term: | Mycobacterium |
| AGROVOC Term: | Antimicrobials |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13063 |
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