Citation
Irnada Syuhaidah Mohd Kamal, . and Noraswati Mohd Nor Rashid, . and Maizirwan Mel, . (2011) A cryopreservation method of Scizophyllum commune local strains. [Proceedings Paper]
Abstract
Schizophyllan commune isolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains wheat corn and paddy. The grains were incubated for 7 days at room temperature. After the grains were fully covered with mycelia they were place into a cryo-vial with three different treatments 10 glycerol sterile distilled water and no cryoprotectants. All of the treatments were frozen in liquid nitrogen for 7 days followed by thawing cooling at 30C for 30 min. The grains were inoculated onto PDA agar to observe the viability rate of survival of the seeds. The radius of each grain with different treatments was measured. Results showed that all treatments showed 100 viability after 48 hours and wheat with no cryoprotectants showed the highest growth rate. This indicated that the grains have mycelial protective properties. In this study a cryopreservation process was simplified as opposed to the conventional method in which 10 glycerol was used as the cryoprotectant. This study promotes an alternative method for cryopreservation which is more economical as no cryoprotectants were required.
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Abstract
Schizophyllan commune isolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains wheat corn and paddy. The grains were incubated for 7 days at room temperature. After the grains were fully covered with mycelia they were place into a cryo-vial with three different treatments 10 glycerol sterile distilled water and no cryoprotectants. All of the treatments were frozen in liquid nitrogen for 7 days followed by thawing cooling at 30C for 30 min. The grains were inoculated onto PDA agar to observe the viability rate of survival of the seeds. The radius of each grain with different treatments was measured. Results showed that all treatments showed 100 viability after 48 hours and wheat with no cryoprotectants showed the highest growth rate. This indicated that the grains have mycelial protective properties. In this study a cryopreservation process was simplified as opposed to the conventional method in which 10 glycerol was used as the cryoprotectant. This study promotes an alternative method for cryopreservation which is more economical as no cryoprotectants were required.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Schizophyllum commune |
| AGROVOC Term: | Plantation crops |
| AGROVOC Term: | Cryopreservation |
| AGROVOC Term: | Spawn fungi |
| AGROVOC Term: | Paddy |
| AGROVOC Term: | Corn maize |
| AGROVOC Term: | Wheats |
| AGROVOC Term: | Temperature |
| AGROVOC Term: | Glycerol |
| AGROVOC Term: | Cryoprotectants |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13067 |
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