Citation
Noriha Mat Amin, . and Siti Noraini Bunawan, . and Noor Aseha Mohd Yahya, . and Marzukhi Hashim, . (2011) Preliminary study : Chitinase activity from Bacillus SP 289. [Proceedings Paper]
Abstract
Bacillus SP 289 previously isolated from rhizosphere soil of lowland rice in Malaysia was found to produce chitinase which caused growth inhibition of Rhizoctonia solani mycelium. The chitinase activity increased with time from 1 to 6 days of incubation in which day 6 gave the highest activity i.e. at 0.0168 U/mL. The enzyme was then partially purified using gradient ammonium sulphate NH‚„‚‚SO‚„ fractionation to determine the best saturation that can precipitated the highest amount of chitinase. Results showed that precipitation with 0-30 of NH‚„‚‚SO‚„ saturation gave the highest chitinase activity i.e. 0.1315 U/mL followed by 0.1079 U/mL at fraction 30-50 NH‚„‚‚SO‚„ saturation. The lowest chitinase activity was detected at fraction 70-100 NH‚„‚‚SO‚„ saturation For the amount of total protein results showed that 50-70 of NH‚„‚‚SO‚„ saturation precipitated the highest amount of total protein 93.41IJg/mL while the lowest protein concentration was found at fraction 70-100 of NH‚„‚‚SO‚„saturation. One hundred microlitres of each precipitated protein fractions from various NH‚„‚‚SO‚„ saturation gradients were then tested against R. solani for its possible antifungal activity. Based on the antifungal assay results all fractions of precipitated protein with high chitinase activity showed inhibition of R. solani mycelial growth except for fraction 70-100 of NH‚„‚‚SO‚„ saturation. This indicates that chitinase may contribute to the antifungal properties of Bacillus SP 289 which can potentially be used as antifungal protein for controlling the rice sheath blight pathogen R. solani.
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Abstract
Bacillus SP 289 previously isolated from rhizosphere soil of lowland rice in Malaysia was found to produce chitinase which caused growth inhibition of Rhizoctonia solani mycelium. The chitinase activity increased with time from 1 to 6 days of incubation in which day 6 gave the highest activity i.e. at 0.0168 U/mL. The enzyme was then partially purified using gradient ammonium sulphate NH‚„‚‚SO‚„ fractionation to determine the best saturation that can precipitated the highest amount of chitinase. Results showed that precipitation with 0-30 of NH‚„‚‚SO‚„ saturation gave the highest chitinase activity i.e. 0.1315 U/mL followed by 0.1079 U/mL at fraction 30-50 NH‚„‚‚SO‚„ saturation. The lowest chitinase activity was detected at fraction 70-100 NH‚„‚‚SO‚„ saturation For the amount of total protein results showed that 50-70 of NH‚„‚‚SO‚„ saturation precipitated the highest amount of total protein 93.41IJg/mL while the lowest protein concentration was found at fraction 70-100 of NH‚„‚‚SO‚„saturation. One hundred microlitres of each precipitated protein fractions from various NH‚„‚‚SO‚„ saturation gradients were then tested against R. solani for its possible antifungal activity. Based on the antifungal assay results all fractions of precipitated protein with high chitinase activity showed inhibition of R. solani mycelial growth except for fraction 70-100 of NH‚„‚‚SO‚„ saturation. This indicates that chitinase may contribute to the antifungal properties of Bacillus SP 289 which can potentially be used as antifungal protein for controlling the rice sheath blight pathogen R. solani.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Bacillus |
| AGROVOC Term: | Gram positive bacteria |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Paddy soil |
| AGROVOC Term: | Incubation of microorganisms |
| AGROVOC Term: | Chitinase |
| AGROVOC Term: | Growth inhibition |
| AGROVOC Term: | Rhizoctonia solani |
| AGROVOC Term: | Enzymatic activity |
| AGROVOC Term: | Antifungal properties |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13068 |
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