Citation
Norazila Yusoff, . and Noor Azlina Ibrahim, . (2011) Thermostable serine protease from thermophilic Bacillus subtilis 50A. [Proceedings Paper]
Abstract
An extracellular thermostable protease producer has been successfully isolated from Lojing hotspring Kelantan Malaysia. The isolate was identified as Bacillus subtilis by 16s rRNA analysis. Effect of temperature on the enzyme activity and stability was investigated. The crude protease displayed the maximal activity at 70C. The thermostability profile exhibited that the protease was very stable at 50C and the activity retained 89 of its original activity after heat treatment at 60C for 30 min. The enzyme activity was totally inhibited by phenylmethyl sulphonyl fluoride PMSF suggested that the protease from B. subtilis 50a belongs to the family of serine protease. From the results it showed that B. subtilis 50a is a good extracellular protease producer at high temperature and thus making it as promising thermostable serine protease producer forindustrial use.
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Abstract
An extracellular thermostable protease producer has been successfully isolated from Lojing hotspring Kelantan Malaysia. The isolate was identified as Bacillus subtilis by 16s rRNA analysis. Effect of temperature on the enzyme activity and stability was investigated. The crude protease displayed the maximal activity at 70C. The thermostability profile exhibited that the protease was very stable at 50C and the activity retained 89 of its original activity after heat treatment at 60C for 30 min. The enzyme activity was totally inhibited by phenylmethyl sulphonyl fluoride PMSF suggested that the protease from B. subtilis 50a belongs to the family of serine protease. From the results it showed that B. subtilis 50a is a good extracellular protease producer at high temperature and thus making it as promising thermostable serine protease producer forindustrial use.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Bacillus subtilis |
| AGROVOC Term: | Proteases |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Microbiological analysis |
| AGROVOC Term: | Water springs |
| AGROVOC Term: | Temperature |
| AGROVOC Term: | Heat treatment |
| AGROVOC Term: | Enzyme activity |
| AGROVOC Term: | Proteases |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13070 |
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