Citation
Sheril Norliana Suhaimi, . and Nur Amelia Azreen Adnan, . and Phang Lai Yee, . (2011) Improved screening method of glycerol-fermenting bacteria for bioethanol production. [Proceedings Paper]
Abstract
The study screening method was able to qualitatively determine the ethanol production of isolated strains. It was improved based on enzymatic oxidation of ethanol correlated to reduction of 2 6-dichlorophenol-indophenol dye that resulted in formation of yellow zone. The formation of decolourized zone was greatly affected by amount of dye reaction mixture and alcohol dehydrogenase used in the assay. Therefore these parameters were optimized to obtain optimal color reduction in presence of ethanol. Many isolates were able to grow in glycerol-containing media. These isolates were subsequently subjected to improved enzymatic assay for screening of ethanol producing bacteria. Potential ethanol-producing bacteria were selected based on the diameter of clear zone. Ethanol produced by the selected isolates was quantified through anaerobic batch fermentation. After 72 h of incubation diameter of clear zone achieved by strain AM 108 was 0.3 cm and ethanol production is 0.629 g/L which is comparable to Enterobacter aerogenes HU101 control known as glycerol-fermenting bacteria for ethanol production. In comparison strain C14 showed lower ethanol producer with the diameter of clear zone is about 0.175 cm and ethanol production is 0.214 g/L. Strain AM 108 is a potential bioethanol-producing bacteria utilizing glycerol as carbon source as ethanol produced was comparable to control.
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Abstract
The study screening method was able to qualitatively determine the ethanol production of isolated strains. It was improved based on enzymatic oxidation of ethanol correlated to reduction of 2 6-dichlorophenol-indophenol dye that resulted in formation of yellow zone. The formation of decolourized zone was greatly affected by amount of dye reaction mixture and alcohol dehydrogenase used in the assay. Therefore these parameters were optimized to obtain optimal color reduction in presence of ethanol. Many isolates were able to grow in glycerol-containing media. These isolates were subsequently subjected to improved enzymatic assay for screening of ethanol producing bacteria. Potential ethanol-producing bacteria were selected based on the diameter of clear zone. Ethanol produced by the selected isolates was quantified through anaerobic batch fermentation. After 72 h of incubation diameter of clear zone achieved by strain AM 108 was 0.3 cm and ethanol production is 0.629 g/L which is comparable to Enterobacter aerogenes HU101 control known as glycerol-fermenting bacteria for ethanol production. In comparison strain C14 showed lower ethanol producer with the diameter of clear zone is about 0.175 cm and ethanol production is 0.214 g/L. Strain AM 108 is a potential bioethanol-producing bacteria utilizing glycerol as carbon source as ethanol produced was comparable to control.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Screening tests |
| AGROVOC Term: | Fermentation |
| AGROVOC Term: | Incubation of microorganisms |
| AGROVOC Term: | Enterobacter aerogenes |
| AGROVOC Term: | Glycerol |
| AGROVOC Term: | Ethanol |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13072 |
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