Citation
Noraini Omar, . and Md Sabri Mohd Yusoff, . (2011) Characterisation and identification of outer membrane protein OMP of Vibrio alginolyticus. [Proceedings Paper]
Abstract
The present study was conducted to characterise and identify the outer membrane protein OMP of Vibrio alginolyticus. The OMP of V. alginolyticus was extracted and characterised by SDS-PAGE and further identified for the antigenic protein by using Western blotting. The membrane protein mainly distributed in the range between 15 to 200 kDa. They were located approximately at 200 90 80 75 65 60 40 33 30 25 and 20 kDa. The result of the Western blot indicated that the most antigenic proteins were located approximately at 200 65 60 and 33 kDa. OMP of 65 kDa and 33 kDa were selected for sequencing and it could be used as the effective vaccine candidates against infection caused by V. alginolyticus.
Download File
Full text available from:
|
Abstract
The present study was conducted to characterise and identify the outer membrane protein OMP of Vibrio alginolyticus. The OMP of V. alginolyticus was extracted and characterised by SDS-PAGE and further identified for the antigenic protein by using Western blotting. The membrane protein mainly distributed in the range between 15 to 200 kDa. They were located approximately at 200 90 80 75 65 60 40 33 30 25 and 20 kDa. The result of the Western blot indicated that the most antigenic proteins were located approximately at 200 65 60 and 33 kDa. OMP of 65 kDa and 33 kDa were selected for sequencing and it could be used as the effective vaccine candidates against infection caused by V. alginolyticus.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Vibrio |
| AGROVOC Term: | Pathogens |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Sds-page |
| AGROVOC Term: | Western blotting |
| AGROVOC Term: | Proteins |
| AGROVOC Term: | Lipids |
| AGROVOC Term: | Antigens |
| AGROVOC Term: | Vaccines |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13076 |
Actions (login required)
 |
View Item |
