Citation
Wong Clemente Michael Vui Ling, . and Yip Kiat Mei, . and Md Amzad Hossain, . (2011) Characterisation of bioactive compounds from tropics and antarctic bacteria. [Proceedings Paper]
Abstract
The aim of the study was to isolate the bioactive compounds responsible for antimicrobial activity from Antarctic and tropical soil bacteria. Thirteen and one bacteria strains isolated from tropical soil in Sabah and the Antarctic respectively exhibited antimicrobial activities against several human pathogens. Identities of these bacteria were confirmed via their 16S rDNA sequences. Secondary metabolite produced by the Antarctic soil bacterium WEA1 Pseudomonas corrugata was extracted by using liquid-liquid extraction and purified with thin layer chromatography. Three fractions obtained from liquid-liquid extraction exhibited antagonism activity against pathogen Enterobacter cloacae 22X were Hexane dichloromethane and chloroform fractions. A white amorphous compound from Hexane fraction was obtained and the structure was elucidated as oleanolic acid. An apparently loss of its antimicrobial activity for isolated oleanolic acid highlighted probability of the synergistic interactions among other substances produced by the bacterium that led for antimicrobial activity.
Download File
Full text available from:
|
Abstract
The aim of the study was to isolate the bioactive compounds responsible for antimicrobial activity from Antarctic and tropical soil bacteria. Thirteen and one bacteria strains isolated from tropical soil in Sabah and the Antarctic respectively exhibited antimicrobial activities against several human pathogens. Identities of these bacteria were confirmed via their 16S rDNA sequences. Secondary metabolite produced by the Antarctic soil bacterium WEA1 Pseudomonas corrugata was extracted by using liquid-liquid extraction and purified with thin layer chromatography. Three fractions obtained from liquid-liquid extraction exhibited antagonism activity against pathogen Enterobacter cloacae 22X were Hexane dichloromethane and chloroform fractions. A white amorphous compound from Hexane fraction was obtained and the structure was elucidated as oleanolic acid. An apparently loss of its antimicrobial activity for isolated oleanolic acid highlighted probability of the synergistic interactions among other substances produced by the bacterium that led for antimicrobial activity.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Tropical soils |
| AGROVOC Term: | Antarctica |
| AGROVOC Term: | Antimicrobial properties |
| AGROVOC Term: | Antagonism |
| AGROVOC Term: | Pathogens |
| AGROVOC Term: | Enterobacter cloacae |
| AGROVOC Term: | Chloroform |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13077 |
Actions (login required)
 |
View Item |
