Citation
Aziyah Abdul Aziz, . and Story Rachel John, . and Sharifah Aminah Syed Mohamad, . and Foong Mohd Faiz Abdullah, . (2011) Growth-phase dependent acid tolerance response in Shigellaflexneri strain 307. [Proceedings Paper]
Abstract
Acid protection mechanism is a characteristic of microorganisms that causes gastrointestinal diseases in human and animals. Shigellaflexneri strain 307 SF307 a clinical isolate from Malaysia is able to demonstrate growth-phase dependent acid resistance AR when challenged for two hours in nutrient broth pH o2.5. As the cells are beginning to enter the stationary phase the degree of acid resistance increases percentage log‚�‚ survival late exponential phase cells 20.6117.95; percentage log10 survival late stationary phase 70.900.47. Mid-exponential cells of SF307 adapted to nutrient broth pH 4.5 prior to challenging in extreme acidic environment pH o3.0 are able to survive for at least two hours in vitropercentage log‚�‚ survival 47.805.12. No viable counts were detected after one hour incubation for cells that were treated with 40 g/mL chloramphenicol. Most current studies focused on the resistance properties of Shigella spp. and less is known regarding the tolerance response of this microorganism. This study aims to characterize the growth phase dependent acid resistance mechanism and the acid tolerance response of S. flexneri and the proteins that governed this response.
Download File
Full text available from:
|
Abstract
Acid protection mechanism is a characteristic of microorganisms that causes gastrointestinal diseases in human and animals. Shigellaflexneri strain 307 SF307 a clinical isolate from Malaysia is able to demonstrate growth-phase dependent acid resistance AR when challenged for two hours in nutrient broth pH o2.5. As the cells are beginning to enter the stationary phase the degree of acid resistance increases percentage log‚�‚ survival late exponential phase cells 20.6117.95; percentage log10 survival late stationary phase 70.900.47. Mid-exponential cells of SF307 adapted to nutrient broth pH 4.5 prior to challenging in extreme acidic environment pH o3.0 are able to survive for at least two hours in vitropercentage log‚�‚ survival 47.805.12. No viable counts were detected after one hour incubation for cells that were treated with 40 g/mL chloramphenicol. Most current studies focused on the resistance properties of Shigella spp. and less is known regarding the tolerance response of this microorganism. This study aims to characterize the growth phase dependent acid resistance mechanism and the acid tolerance response of S. flexneri and the proteins that governed this response.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Shigella |
| AGROVOC Term: | Gastrointestinal diseases |
| AGROVOC Term: | Microorganisms |
| AGROVOC Term: | Culture of microorganisms |
| AGROVOC Term: | Incubation of microorganisms |
| AGROVOC Term: | Survival |
| AGROVOC Term: | Growth |
| AGROVOC Term: | Inhibition |
| AGROVOC Term: | Proteins |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13079 |
Actions (login required)
 |
View Item |
