Isolation of ligninase producing Basidiomycetes from local terresterial

Tay Amelia ChuFer, . and Yong Suh Kuan, . and Tee Jiun Tsong, . and Ong Lisa Gaik Ai, . and Chan Mun Yee, . and Hii Siew Ling, . (2011) Isolation of ligninase producing Basidiomycetes from local terresterial. [Proceedings Paper]

Synthetic dyes have replaced the use of natural dye in the textile industry. Generally the synthesized dyes are more colourful easy to manufacture resistant to fading and have a diverse range of colour. Despite all these mentioned advantages this group of dye has caused water pollution in Malaysia especially in the state of Terengganu and Kelantan. They have caused aesthetic pollution of waterways due to the presence of dyes leached from textile factories since they are visible even in trace amounts. Ligninase produced by basidiomycetes is able to degrade azo heterocyclic reactive and polymeric dyes which have been found abundant in the wastewater. This enzyme is further subdivided into laccase lignin peroxidase and manganese peroxidase. Thus fungal isolated from Perak and Selangor were tested for ligninolytic enzyme production on plates containing 3 commercial dyes. Of the tested isolates a total of 8 13 and 11 isolates showed positive for 2 2;-azino-bis3-ethylbenzothiazoline-6-sulphonic acid ABTS Remazol Brilliant Blue R RBBR and guaiacol respectively. Isolates positive for all three dyes were subjected to morphological identification by using lactophenol staining.

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Abstract

Synthetic dyes have replaced the use of natural dye in the textile industry. Generally the synthesized dyes are more colourful easy to manufacture resistant to fading and have a diverse range of colour. Despite all these mentioned advantages this group of dye has caused water pollution in Malaysia especially in the state of Terengganu and Kelantan. They have caused aesthetic pollution of waterways due to the presence of dyes leached from textile factories since they are visible even in trace amounts. Ligninase produced by basidiomycetes is able to degrade azo heterocyclic reactive and polymeric dyes which have been found abundant in the wastewater. This enzyme is further subdivided into laccase lignin peroxidase and manganese peroxidase. Thus fungal isolated from Perak and Selangor were tested for ligninolytic enzyme production on plates containing 3 commercial dyes. Of the tested isolates a total of 8 13 and 11 isolates showed positive for 2 2;-azino-bis3-ethylbenzothiazoline-6-sulphonic acid ABTS Remazol Brilliant Blue R RBBR and guaiacol respectively. Isolates positive for all three dyes were subjected to morphological identification by using lactophenol staining.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Dyes
AGROVOC Term:	Basidiomycetes
AGROVOC Term:	Fungi
AGROVOC Term:	Isolation
AGROVOC Term:	Enzymes
AGROVOC Term:	Ligninolytic microorganisms
AGROVOC Term:	Laccase
AGROVOC Term:	Dyes
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13088

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