Citation
Yee Siew Fung, . and Eng Lily, . and Evenni Poili, . and Julia Lamdin, . (2011) Isolation of tungro viruses using different methods of virus purification A preliminary study. [Proceedings Paper]
Abstract
Isolation and purification of tungro viruses can be carried out using an ultracentrifuge or a high speed centrifuge. Rice tungro spherical virus RTSV and rice tungro bacilliform virus RTBV were multiplied in ;Taichung Native l; TN1 rice cultivar which has been fed to the green leafhopper Nephotettix virescens previously reared on TN1 infected with both tungro viruses. Tungro viruses were then separately isolated and purified by ultracentrifugation. RTBV particles purified were about 28 to 40 nm in width and about 130 to 200 nm in length. The diameter of purified RTSV particles was about 20 to 40 nm.
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Abstract
Isolation and purification of tungro viruses can be carried out using an ultracentrifuge or a high speed centrifuge. Rice tungro spherical virus RTSV and rice tungro bacilliform virus RTBV were multiplied in ;Taichung Native l; TN1 rice cultivar which has been fed to the green leafhopper Nephotettix virescens previously reared on TN1 infected with both tungro viruses. Tungro viruses were then separately isolated and purified by ultracentrifugation. RTBV particles purified were about 28 to 40 nm in width and about 130 to 200 nm in length. The diameter of purified RTSV particles was about 20 to 40 nm.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Rice |
| AGROVOC Term: | Cultivars |
| AGROVOC Term: | Disease incidence |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Purification |
| AGROVOC Term: | Ultracentrifugation |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Leafhoppers |
| AGROVOC Term: | Nephotettix virescens |
| AGROVOC Term: | Rice tungro bacilliform virus |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13089 |
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